Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1135 and Shew_1136 are separated by 60 nucleotides Shew_1136 and Shew_1137 are separated by 81 nucleotides Shew_1137 and Shew_1138 are separated by 35 nucleotides
Shew_1135: Shew_1135 - protein of unknown function YGGT (RefSeq), at 1,310,024 to 1,310,572
_1135
Shew_1136: Shew_1136 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 1,310,633 to 1,311,451
_1136
Shew_1137: Shew_1137 - alanine racemase domain-containing protein (RefSeq), at 1,311,533 to 1,312,234
_1137
Shew_1138: Shew_1138 - twitching motility protein (RefSeq), at 1,312,270 to 1,313,307
_1138
Position (kb)
1311
1312
1313 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1310.666 kb on - strand at 1310.700 kb on - strand at 1310.849 kb on + strand, within Shew_1136 at 1311.110 kb on - strand, within Shew_1136 at 1311.303 kb on + strand, within Shew_1136 at 1311.378 kb on + strand at 1311.378 kb on + strand at 1311.378 kb on + strand at 1311.386 kb on - strand at 1311.518 kb on - strand at 1311.518 kb on - strand at 1311.530 kb on + strand at 1311.636 kb on - strand, within Shew_1137 at 1311.764 kb on - strand, within Shew_1137 at 1312.306 kb on - strand at 1312.500 kb on - strand, within Shew_1138 at 1312.531 kb on - strand, within Shew_1138 at 1312.531 kb on - strand, within Shew_1138 at 1312.773 kb on - strand, within Shew_1138 at 1312.821 kb on + strand, within Shew_1138 at 1313.169 kb on + strand, within Shew_1138 at 1313.213 kb on + strand at 1313.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,310,666 - -3.9 1,310,700 - -2.7 1,310,849 + Shew_1136 0.26 -4.5 1,311,110 - Shew_1136 0.58 -6.0 1,311,303 + Shew_1136 0.82 -3.6 1,311,378 + -5.8 1,311,378 + -3.0 1,311,378 + -4.1 1,311,386 - -1.5 1,311,518 - -0.7 1,311,518 - -1.7 1,311,530 + +0.3 1,311,636 - Shew_1137 0.15 +0.9 1,311,764 - Shew_1137 0.33 -0.4 1,312,306 - -0.9 1,312,500 - Shew_1138 0.22 +0.3 1,312,531 - Shew_1138 0.25 +0.7 1,312,531 - Shew_1138 0.25 +0.7 1,312,773 - Shew_1138 0.48 +0.2 1,312,821 + Shew_1138 0.53 +0.9 1,313,169 + Shew_1138 0.87 -0.2 1,313,213 + +0.1 1,313,213 + +0.3
Or see this region's nucleotide sequence