Strain Fitness in Shewanella loihica PV-4 around Shew_1081

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrA and Shew_1080 overlap by 4 nucleotidesShew_1080 and Shew_1081 are separated by 13 nucleotidesShew_1081 and Shew_1082 are separated by 358 nucleotidesShew_1082 and Shew_1083 overlap by 4 nucleotides Shew_1079: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq), at 1,241,698 to 1,244,166 thrA Shew_1080: Shew_1080 - homoserine kinase (RefSeq), at 1,244,163 to 1,245,113 _1080 Shew_1081: Shew_1081 - threonine synthase (RefSeq), at 1,245,127 to 1,246,410 _1081 Shew_1082: Shew_1082 - hypothetical protein (RefSeq), at 1,246,769 to 1,247,065 _1082 Shew_1083: Shew_1083 - apurinic endonuclease Apn1 (RefSeq), at 1,247,062 to 1,247,913 _1083 Position (kb) 1245 1246 1247Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 1244.135 kb on + strandat 1244.159 kb on - strandat 1244.415 kb on + strand, within Shew_1080at 1244.689 kb on - strand, within Shew_1080at 1245.327 kb on + strand, within Shew_1081at 1245.386 kb on - strand, within Shew_1081at 1245.518 kb on + strand, within Shew_1081at 1245.564 kb on + strand, within Shew_1081at 1246.027 kb on - strand, within Shew_1081at 1246.332 kb on + strandat 1246.340 kb on - strandat 1246.377 kb on + strandat 1246.377 kb on + strandat 1246.377 kb on + strandat 1246.385 kb on - strandat 1246.950 kb on - strand, within Shew_1082at 1247.053 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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1,244,135 + -2.9
1,244,159 - -3.7
1,244,415 + Shew_1080 0.26 -2.7
1,244,689 - Shew_1080 0.55 -2.6
1,245,327 + Shew_1081 0.16 -6.2
1,245,386 - Shew_1081 0.20 -2.6
1,245,518 + Shew_1081 0.30 -5.1
1,245,564 + Shew_1081 0.34 -5.1
1,246,027 - Shew_1081 0.70 -3.6
1,246,332 + -6.2
1,246,340 - -3.9
1,246,377 + -0.2
1,246,377 + -1.6
1,246,377 + -1.8
1,246,385 - -0.7
1,246,950 - Shew_1082 0.61 +0.7
1,247,053 - +0.5

Or see this region's nucleotide sequence