Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and Shew_1080 overlap by 4 nucleotides Shew_1080 and Shew_1081 are separated by 13 nucleotides Shew_1081 and Shew_1082 are separated by 358 nucleotides Shew_1082 and Shew_1083 overlap by 4 nucleotides
Shew_1079: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq), at 1,241,698 to 1,244,166
thrA
Shew_1080: Shew_1080 - homoserine kinase (RefSeq), at 1,244,163 to 1,245,113
_1080
Shew_1081: Shew_1081 - threonine synthase (RefSeq), at 1,245,127 to 1,246,410
_1081
Shew_1082: Shew_1082 - hypothetical protein (RefSeq), at 1,246,769 to 1,247,065
_1082
Shew_1083: Shew_1083 - apurinic endonuclease Apn1 (RefSeq), at 1,247,062 to 1,247,913
_1083
Position (kb)
1245
1246
1247 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1244.135 kb on + strand at 1244.159 kb on - strand at 1244.415 kb on + strand, within Shew_1080 at 1244.689 kb on - strand, within Shew_1080 at 1245.327 kb on + strand, within Shew_1081 at 1245.386 kb on - strand, within Shew_1081 at 1245.518 kb on + strand, within Shew_1081 at 1245.564 kb on + strand, within Shew_1081 at 1246.027 kb on - strand, within Shew_1081 at 1246.332 kb on + strand at 1246.340 kb on - strand at 1246.377 kb on + strand at 1246.377 kb on + strand at 1246.377 kb on + strand at 1246.385 kb on - strand at 1246.950 kb on - strand, within Shew_1082 at 1247.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,244,135 + -2.9 1,244,159 - -3.7 1,244,415 + Shew_1080 0.26 -2.7 1,244,689 - Shew_1080 0.55 -2.6 1,245,327 + Shew_1081 0.16 -6.2 1,245,386 - Shew_1081 0.20 -2.6 1,245,518 + Shew_1081 0.30 -5.1 1,245,564 + Shew_1081 0.34 -5.1 1,246,027 - Shew_1081 0.70 -3.6 1,246,332 + -6.2 1,246,340 - -3.9 1,246,377 + -0.2 1,246,377 + -1.6 1,246,377 + -1.8 1,246,385 - -0.7 1,246,950 - Shew_1082 0.61 +0.7 1,247,053 - +0.5
Or see this region's nucleotide sequence