Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0911 and Shew_0912 are separated by 5 nucleotides Shew_0912 and Shew_0913 are separated by 64 nucleotides Shew_0913 and Shew_0914 are separated by 139 nucleotides Shew_0914 and Shew_0915 are separated by 140 nucleotides Shew_0915 and Shew_0916 overlap by 11 nucleotides
Shew_0911: Shew_0911 - hypothetical protein (RefSeq), at 1,033,033 to 1,033,335
_0911
Shew_0912: Shew_0912 - hypothetical protein (RefSeq), at 1,033,341 to 1,033,589
_0912
Shew_0913: Shew_0913 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 1,033,654 to 1,034,076
_0913
Shew_0914: Shew_0914 - NADPH-dependent FMN reductase (RefSeq), at 1,034,216 to 1,034,821
_0914
Shew_0915: Shew_0915 - putative transcriptional regulator (RefSeq), at 1,034,962 to 1,035,618
_0915
Shew_0916: Shew_0916 - pseudouridine synthase (RefSeq), at 1,035,608 to 1,036,318
_0916
Position (kb)
1034
1035 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1033.259 kb on - strand, within Shew_0911 at 1033.261 kb on - strand, within Shew_0911 at 1033.819 kb on + strand, within Shew_0913 at 1033.890 kb on - strand, within Shew_0913 at 1034.177 kb on - strand at 1034.255 kb on - strand at 1034.335 kb on + strand, within Shew_0914 at 1034.343 kb on - strand, within Shew_0914 at 1034.486 kb on + strand, within Shew_0914 at 1034.565 kb on + strand, within Shew_0914 at 1034.615 kb on - strand, within Shew_0914 at 1034.695 kb on + strand, within Shew_0914 at 1035.682 kb on + strand, within Shew_0916
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,033,259 - Shew_0911 0.75 -0.4 1,033,261 - Shew_0911 0.75 +1.1 1,033,819 + Shew_0913 0.39 -0.0 1,033,890 - Shew_0913 0.56 -0.4 1,034,177 - -0.1 1,034,255 - -0.2 1,034,335 + Shew_0914 0.20 +0.5 1,034,343 - Shew_0914 0.21 -0.6 1,034,486 + Shew_0914 0.45 +0.1 1,034,565 + Shew_0914 0.58 +1.1 1,034,615 - Shew_0914 0.66 +2.0 1,034,695 + Shew_0914 0.79 +4.1 1,035,682 + Shew_0916 0.10 -1.5
Or see this region's nucleotide sequence