Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0721 and Shew_0722 are separated by 11 nucleotides Shew_0722 and Shew_0723 are separated by 64 nucleotides Shew_0723 and Shew_0724 are separated by 11 nucleotides
Shew_0721: Shew_0721 - RND family efflux transporter MFP subunit (RefSeq), at 821,137 to 822,399
_0721
Shew_0722: Shew_0722 - hypothetical protein (RefSeq), at 822,411 to 822,647
_0722
Shew_0723: Shew_0723 - ABC transporter-related protein (RefSeq), at 822,712 to 823,434
_0723
Shew_0724: Shew_0724 - hypothetical protein (RefSeq), at 823,446 to 824,756
_0724
Position (kb) 822
823
824 Strain fitness (log2 ratio) -2
-1
0
1
2 at 822.237 kb on + strand, within Shew_0721 at 822.245 kb on - strand, within Shew_0721 at 822.305 kb on + strand at 822.305 kb on + strand at 822.305 kb on + strand at 822.313 kb on - strand at 822.499 kb on - strand, within Shew_0722 at 822.686 kb on - strand at 822.925 kb on - strand, within Shew_0723 at 823.038 kb on - strand, within Shew_0723 at 823.071 kb on - strand, within Shew_0723 at 823.378 kb on - strand at 823.380 kb on + strand at 823.380 kb on + strand at 823.380 kb on + strand at 823.475 kb on + strand at 823.557 kb on - strand at 823.618 kb on - strand, within Shew_0724 at 823.773 kb on - strand, within Shew_0724 at 823.800 kb on - strand, within Shew_0724 at 823.800 kb on - strand, within Shew_0724 at 824.058 kb on + strand, within Shew_0724
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 822,237 + Shew_0721 0.87 +0.1 822,245 - Shew_0721 0.88 -0.8 822,305 + +0.6 822,305 + +2.0 822,305 + -0.1 822,313 - -0.8 822,499 - Shew_0722 0.37 -0.6 822,686 - +0.3 822,925 - Shew_0723 0.29 -0.8 823,038 - Shew_0723 0.45 +0.6 823,071 - Shew_0723 0.50 -1.9 823,378 - -0.2 823,380 + +0.2 823,380 + +2.1 823,380 + -0.1 823,475 + -0.1 823,557 - -0.1 823,618 - Shew_0724 0.13 -1.2 823,773 - Shew_0724 0.25 -0.3 823,800 - Shew_0724 0.27 -2.0 823,800 - Shew_0724 0.27 -0.2 824,058 + Shew_0724 0.47 -0.2
Or see this region's nucleotide sequence