Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2069 and DvMF_2070 are separated by 619 nucleotides DvMF_2070 and DvMF_2071 overlap by 4 nucleotides DvMF_2071 and DvMF_2072 are separated by 74 nucleotides
DvMF_2069: DvMF_2069 - DNA topoisomerase I (RefSeq), at 2,567,011 to 2,569,287
_2069
DvMF_2070: DvMF_2070 - hypothetical protein (RefSeq), at 2,569,907 to 2,571,382
_2070
DvMF_2071: DvMF_2071 - hypothetical protein (RefSeq), at 2,571,379 to 2,572,233
_2071
DvMF_2072: DvMF_2072 - hypothetical protein (RefSeq), at 2,572,308 to 2,573,084
_2072
Position (kb)
2569
2570
2571
2572 Strain fitness (log2 ratio)
-1
0
1 at 2569.370 kb on + strand at 2569.488 kb on - strand at 2569.506 kb on - strand at 2569.597 kb on - strand at 2569.659 kb on + strand at 2569.677 kb on - strand at 2569.677 kb on - strand at 2569.677 kb on - strand at 2569.692 kb on + strand at 2569.721 kb on - strand at 2569.847 kb on - strand at 2570.345 kb on - strand, within DvMF_2070 at 2570.886 kb on + strand, within DvMF_2070 at 2571.036 kb on + strand, within DvMF_2070 at 2571.192 kb on + strand, within DvMF_2070 at 2571.193 kb on - strand, within DvMF_2070 at 2571.292 kb on + strand at 2571.473 kb on + strand, within DvMF_2071 at 2571.488 kb on + strand, within DvMF_2071 at 2571.488 kb on + strand, within DvMF_2071 at 2571.488 kb on + strand, within DvMF_2071 at 2571.687 kb on - strand, within DvMF_2071 at 2571.861 kb on - strand, within DvMF_2071 at 2572.197 kb on + strand at 2572.339 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 2,569,370 + +0.1 2,569,488 - +0.1 2,569,506 - -0.1 2,569,597 - +0.1 2,569,659 + +0.2 2,569,677 - +0.3 2,569,677 - -0.2 2,569,677 - -0.1 2,569,692 + +0.0 2,569,721 - +0.3 2,569,847 - +0.0 2,570,345 - DvMF_2070 0.30 +0.0 2,570,886 + DvMF_2070 0.66 +0.9 2,571,036 + DvMF_2070 0.76 +0.1 2,571,192 + DvMF_2070 0.87 +0.3 2,571,193 - DvMF_2070 0.87 +0.4 2,571,292 + +0.3 2,571,473 + DvMF_2071 0.11 +0.4 2,571,488 + DvMF_2071 0.13 -0.2 2,571,488 + DvMF_2071 0.13 +0.6 2,571,488 + DvMF_2071 0.13 +0.3 2,571,687 - DvMF_2071 0.36 +0.2 2,571,861 - DvMF_2071 0.56 +0.2 2,572,197 + +0.1 2,572,339 + +0.2
Or see this region's nucleotide sequence