Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1968 and DvMF_1969 are separated by 219 nucleotides DvMF_1969 and DvMF_1970 are separated by 474 nucleotides DvMF_1970 and DvMF_R0052 are separated by 445 nucleotides DvMF_R0052 and DvMF_1971 are separated by 155 nucleotides
DvMF_1968: DvMF_1968 - type I phosphodiesterase/nucleotide pyrophosphatase (RefSeq), at 2,432,710 to 2,434,473
_1968
DvMF_1969: DvMF_1969 - 4-oxalocrotonate tautomerase family enzyme (RefSeq), at 2,434,693 to 2,434,896
_1969
DvMF_1970: DvMF_1970 - hypothetical protein (RefSeq), at 2,435,371 to 2,435,865
_1970
DvMF_R0052: DvMF_R0052 - tRNA-Leu (RefSeq), at 2,436,311 to 2,436,397
_R0052
DvMF_1971: DvMF_1971 - CRISPR-associated helicase Cas3 (RefSeq), at 2,436,553 to 2,438,775
_1971
Position (kb)
2435
2436 Strain fitness (log2 ratio)
-1
0
1 at 2434.374 kb on + strand at 2434.374 kb on + strand at 2434.390 kb on - strand at 2434.482 kb on - strand at 2434.745 kb on - strand, within DvMF_1969 at 2435.138 kb on + strand at 2435.143 kb on + strand at 2435.143 kb on + strand at 2435.176 kb on + strand at 2435.176 kb on + strand at 2435.184 kb on - strand at 2435.184 kb on - strand at 2435.212 kb on - strand at 2435.245 kb on - strand at 2435.330 kb on + strand at 2435.330 kb on + strand at 2435.414 kb on + strand at 2435.427 kb on - strand, within DvMF_1970 at 2435.460 kb on + strand, within DvMF_1970 at 2435.463 kb on - strand, within DvMF_1970 at 2435.596 kb on - strand, within DvMF_1970 at 2435.831 kb on - strand at 2435.885 kb on + strand at 2435.885 kb on + strand at 2435.895 kb on + strand at 2435.913 kb on - strand at 2436.095 kb on + strand at 2436.426 kb on + strand at 2436.434 kb on - strand at 2436.443 kb on - strand at 2436.470 kb on + strand at 2436.478 kb on - strand at 2436.762 kb on + strand at 2436.773 kb on + strand at 2436.845 kb on + strand, within DvMF_1971 at 2436.853 kb on - strand, within DvMF_1971
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 2,434,374 + -0.4 2,434,374 + -0.5 2,434,390 - -0.6 2,434,482 - -0.4 2,434,745 - DvMF_1969 0.25 -0.3 2,435,138 + -0.5 2,435,143 + -0.1 2,435,143 + -1.7 2,435,176 + -0.4 2,435,176 + -0.7 2,435,184 - -1.1 2,435,184 - -0.3 2,435,212 - -0.7 2,435,245 - -0.5 2,435,330 + -0.7 2,435,330 + -0.9 2,435,414 + -0.3 2,435,427 - DvMF_1970 0.11 -0.4 2,435,460 + DvMF_1970 0.18 -0.6 2,435,463 - DvMF_1970 0.19 -0.6 2,435,596 - DvMF_1970 0.45 -0.2 2,435,831 - -0.7 2,435,885 + -1.7 2,435,885 + -0.4 2,435,895 + -0.4 2,435,913 - -0.6 2,436,095 + -0.3 2,436,426 + -0.5 2,436,434 - -0.3 2,436,443 - -0.2 2,436,470 + -0.3 2,436,478 - -0.5 2,436,762 + -0.1 2,436,773 + -0.4 2,436,845 + DvMF_1971 0.13 -0.7 2,436,853 - DvMF_1971 0.13 -0.0
Or see this region's nucleotide sequence