Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0117 and DvMF_0118 overlap by 1 nucleotides DvMF_0118 and DvMF_0119 are separated by 6 nucleotides DvMF_0119 and ispG are separated by 428 nucleotides
DvMF_0117: DvMF_0117 - ABC-3 protein (RefSeq), at 127,653 to 128,483
_0117
DvMF_0118: DvMF_0118 - ABC transporter related (RefSeq), at 128,483 to 129,409
_0118
DvMF_0119: DvMF_0119 - periplasmic solute binding protein (RefSeq), at 129,416 to 130,450
_0119
DvMF_0120: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq), at 130,879 to 131,970
ispG
Position (kb) 129
130
131 Strain fitness (log2 ratio) -2
-1
0
1 at 128.702 kb on + strand, within DvMF_0118 at 128.712 kb on - strand, within DvMF_0118 at 128.734 kb on - strand, within DvMF_0118 at 128.784 kb on - strand, within DvMF_0118 at 128.784 kb on - strand, within DvMF_0118 at 128.802 kb on + strand, within DvMF_0118 at 129.442 kb on - strand at 129.493 kb on - strand at 129.582 kb on + strand, within DvMF_0119 at 129.676 kb on + strand, within DvMF_0119 at 129.684 kb on - strand, within DvMF_0119 at 129.767 kb on + strand, within DvMF_0119 at 129.767 kb on + strand, within DvMF_0119 at 129.843 kb on + strand, within DvMF_0119 at 130.103 kb on - strand, within DvMF_0119 at 130.431 kb on + strand at 130.432 kb on + strand at 130.566 kb on + strand at 130.717 kb on + strand at 130.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 128,702 + DvMF_0118 0.24 +0.7 128,712 - DvMF_0118 0.25 +1.0 128,734 - DvMF_0118 0.27 -0.6 128,784 - DvMF_0118 0.32 -0.4 128,784 - DvMF_0118 0.32 -1.7 128,802 + DvMF_0118 0.34 -0.5 129,442 - -1.3 129,493 - +0.5 129,582 + DvMF_0119 0.16 -0.7 129,676 + DvMF_0119 0.25 +0.2 129,684 - DvMF_0119 0.26 -0.6 129,767 + DvMF_0119 0.34 +0.0 129,767 + DvMF_0119 0.34 -0.4 129,843 + DvMF_0119 0.41 -0.3 130,103 - DvMF_0119 0.66 -0.3 130,431 + +0.4 130,432 + +0.1 130,566 + -0.1 130,717 + -0.4 130,731 - -1.9
Or see this region's nucleotide sequence