Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2299 and Shew_2300 are separated by 151 nucleotides Shew_2300 and Shew_2301 are separated by 152 nucleotides Shew_2301 and Shew_2302 are separated by 188 nucleotides
Shew_2299: Shew_2299 - DNA topoisomerase III (RefSeq), at 2,692,559 to 2,694,571
_2299
Shew_2300: Shew_2300 - hypothetical protein (RefSeq), at 2,694,723 to 2,694,998
_2300
Shew_2301: Shew_2301 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 2,695,151 to 2,697,268
_2301
Shew_2302: Shew_2302 - amidophosphoribosyltransferase (RefSeq), at 2,697,457 to 2,698,971
_2302
Position (kb)
2695
2696
2697
2698 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2694.348 kb on + strand, within Shew_2299 at 2694.955 kb on + strand, within Shew_2300 at 2695.425 kb on + strand, within Shew_2301 at 2695.458 kb on + strand, within Shew_2301 at 2695.667 kb on - strand, within Shew_2301 at 2695.667 kb on - strand, within Shew_2301 at 2695.771 kb on - strand, within Shew_2301 at 2696.252 kb on - strand, within Shew_2301 at 2696.933 kb on - strand, within Shew_2301 at 2696.976 kb on - strand, within Shew_2301 at 2697.378 kb on - strand at 2697.870 kb on + strand, within Shew_2302 at 2697.989 kb on + strand, within Shew_2302
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 2,694,348 + Shew_2299 0.89 -0.5 2,694,955 + Shew_2300 0.84 -1.3 2,695,425 + Shew_2301 0.13 -1.3 2,695,458 + Shew_2301 0.14 +0.1 2,695,667 - Shew_2301 0.24 +0.5 2,695,667 - Shew_2301 0.24 +1.5 2,695,771 - Shew_2301 0.29 +0.8 2,696,252 - Shew_2301 0.52 +1.9 2,696,933 - Shew_2301 0.84 -1.6 2,696,976 - Shew_2301 0.86 +0.2 2,697,378 - +0.5 2,697,870 + Shew_2302 0.27 -3.5 2,697,989 + Shew_2302 0.35 -4.0
Or see this region's nucleotide sequence