Strain Fitness in Shewanella loihica PV-4 around Shew_2200

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_2199 and Shew_2200 are separated by 64 nucleotidesShew_2200 and hisH overlap by 4 nucleotideshisH and Shew_2202 overlap by 4 nucleotides Shew_2199: Shew_2199 - histidinol-phosphate aminotransferase (RefSeq), at 2,559,814 to 2,560,896 _2199 Shew_2200: Shew_2200 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,560,961 to 2,562,031 _2200 Shew_2201: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,562,028 to 2,562,702 hisH Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436 _2202 Position (kb) 2560 2561 2562 2563Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 2560.312 kb on + strand, within Shew_2199at 2560.511 kb on + strand, within Shew_2199at 2560.519 kb on - strand, within Shew_2199at 2560.638 kb on + strand, within Shew_2199at 2560.881 kb on + strandat 2561.293 kb on + strand, within Shew_2200at 2561.399 kb on + strand, within Shew_2200at 2561.417 kb on + strand, within Shew_2200at 2561.425 kb on - strand, within Shew_2200at 2561.499 kb on + strand, within Shew_2200at 2561.750 kb on + strand, within Shew_2200at 2561.758 kb on - strand, within Shew_2200at 2562.347 kb on + strand, within hisHat 2562.722 kb on + strandat 2562.800 kb on + strand, within Shew_2202

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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2,560,312 + Shew_2199 0.46 -1.7
2,560,511 + Shew_2199 0.64 -3.5
2,560,519 - Shew_2199 0.65 -4.2
2,560,638 + Shew_2199 0.76 -2.9
2,560,881 + -1.5
2,561,293 + Shew_2200 0.31 -4.1
2,561,399 + Shew_2200 0.41 -4.6
2,561,417 + Shew_2200 0.43 -3.3
2,561,425 - Shew_2200 0.43 -4.7
2,561,499 + Shew_2200 0.50 -7.2
2,561,750 + Shew_2200 0.74 -6.9
2,561,758 - Shew_2200 0.74 -3.0
2,562,347 + hisH Shew_2201 0.47 -6.8
2,562,722 + -4.6
2,562,800 + Shew_2202 0.14 -2.9

Or see this region's nucleotide sequence