Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2087 and Shew_2088 are separated by 15 nucleotides Shew_2088 and Shew_2089 overlap by 24 nucleotides Shew_2089 and Shew_2090 are separated by 3 nucleotides Shew_2090 and thiH are separated by 102 nucleotides
Shew_2087: Shew_2087 - hypothetical protein (RefSeq), at 2,414,398 to 2,415,549
_2087
Shew_2088: Shew_2088 - LysR family transcriptional regulator (RefSeq), at 2,415,565 to 2,416,476
_2088
Shew_2089: Shew_2089 - SrpA-related protein (RefSeq), at 2,416,453 to 2,417,850
_2089
Shew_2090: Shew_2090 - methyl-accepting chemotaxis protein (RefSeq), at 2,417,854 to 2,418,123
_2090
Shew_2091: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,418,226 to 2,419,344
thiH
Position (kb) 2416
2417
2418 Strain fitness (log2 ratio) -1
0
1
2 at 2415.492 kb on + strand at 2416.197 kb on - strand, within Shew_2088 at 2416.450 kb on + strand at 2416.888 kb on - strand, within Shew_2089 at 2417.080 kb on - strand, within Shew_2089 at 2417.296 kb on - strand, within Shew_2089 at 2418.052 kb on + strand, within Shew_2090
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 2,415,492 + -0.0 2,416,197 - Shew_2088 0.69 +1.8 2,416,450 + -0.3 2,416,888 - Shew_2089 0.31 +0.2 2,417,080 - Shew_2089 0.45 -1.4 2,417,296 - Shew_2089 0.60 -0.8 2,418,052 + Shew_2090 0.73 +1.1
Or see this region's nucleotide sequence