Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1774 and Shew_1775 are separated by 173 nucleotides Shew_1775 and Shew_1776 overlap by 1 nucleotides Shew_1776 and Shew_1777 are separated by 453 nucleotides
Shew_1774: Shew_1774 - excinuclease ATPase subunit (RefSeq), at 2,029,126 to 2,029,623
_1774
Shew_1775: Shew_1775 - acetolactate synthase 3 regulatory subunit (RefSeq), at 2,029,797 to 2,030,291
_1775
Shew_1776: Shew_1776 - acetolactate synthase 3 catalytic subunit (RefSeq), at 2,030,291 to 2,032,012
_1776
Shew_1777: Shew_1777 - Bcr/CflA subfamily drug resistance transporter (RefSeq), at 2,032,466 to 2,033,689
_1777
Position (kb)
2030
2031
2032
2033 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2029.298 kb on - strand, within Shew_1774 at 2029.841 kb on - strand at 2029.922 kb on + strand, within Shew_1775 at 2030.173 kb on - strand, within Shew_1775 at 2030.360 kb on - strand at 2030.654 kb on - strand, within Shew_1776 at 2031.228 kb on - strand, within Shew_1776 at 2031.336 kb on - strand, within Shew_1776 at 2031.454 kb on + strand, within Shew_1776
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 2,029,298 - Shew_1774 0.35 +0.4 2,029,841 - -4.4 2,029,922 + Shew_1775 0.25 -3.2 2,030,173 - Shew_1775 0.76 -4.3 2,030,360 - -6.0 2,030,654 - Shew_1776 0.21 -3.5 2,031,228 - Shew_1776 0.54 -3.4 2,031,336 - Shew_1776 0.61 -3.7 2,031,454 + Shew_1776 0.68 -4.9
Or see this region's nucleotide sequence