Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0518 and Shew_0519 are separated by 78 nucleotides Shew_0519 and Shew_0520 are separated by 224 nucleotides Shew_0520 and metL are separated by 19 nucleotides
Shew_0518: Shew_0518 - hypothetical protein (RefSeq), at 587,818 to 588,558
_0518
Shew_0519: Shew_0519 - transcriptional repressor protein MetJ (RefSeq), at 588,637 to 588,951
_0519
Shew_0520: Shew_0520 - O-succinylhomoserine (thiol)-lyase (RefSeq), at 589,176 to 590,342
_0520
Shew_0521: metL - bifunctional aspartate kinase II/homoserine dehydrogenase II (RefSeq), at 590,362 to 592,755
metL
Position (kb) 589
590
591 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 588.520 kb on - strand at 588.541 kb on + strand at 588.809 kb on + strand, within Shew_0519 at 588.955 kb on - strand at 588.955 kb on - strand at 589.201 kb on + strand at 589.444 kb on - strand, within Shew_0520 at 589.609 kb on - strand, within Shew_0520 at 589.711 kb on - strand, within Shew_0520 at 589.794 kb on - strand, within Shew_0520 at 589.858 kb on + strand, within Shew_0520 at 589.866 kb on - strand, within Shew_0520
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 588,520 - -2.6 588,541 + -3.3 588,809 + Shew_0519 0.55 +1.9 588,955 - -1.8 588,955 - -4.1 589,201 + -4.5 589,444 - Shew_0520 0.23 -4.1 589,609 - Shew_0520 0.37 -4.1 589,711 - Shew_0520 0.46 -5.5 589,794 - Shew_0520 0.53 -4.7 589,858 + Shew_0520 0.58 -5.4 589,866 - Shew_0520 0.59 -3.3
Or see this region's nucleotide sequence