Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2559 and DvMF_2560 are separated by 243 nucleotides DvMF_2560 and DvMF_2561 are separated by 117 nucleotides DvMF_2561 and DvMF_2562 overlap by 4 nucleotides DvMF_2562 and DvMF_R0070 are separated by 123 nucleotides
DvMF_2559: DvMF_2559 - hypothetical protein (RefSeq), at 3,235,070 to 3,235,828
_2559
DvMF_2560: DvMF_2560 - transcriptional regulator, AraC family (RefSeq), at 3,236,072 to 3,237,073
_2560
DvMF_2561: DvMF_2561 - hypothetical protein (RefSeq), at 3,237,191 to 3,237,337
_2561
DvMF_2562: DvMF_2562 - ThiJ/PfpI domain protein (RefSeq), at 3,237,334 to 3,237,930
_2562
DvMF_R0070: DvMF_R0070 - tRNA-Glu (RefSeq), at 3,238,054 to 3,238,130
_R0070
Position (kb)
3236
3237
3238 Strain fitness (log2 ratio)
-2
-1
0
1 at 3235.216 kb on - strand, within DvMF_2559 at 3235.266 kb on - strand, within DvMF_2559 at 3235.325 kb on + strand, within DvMF_2559 at 3235.325 kb on + strand, within DvMF_2559 at 3235.333 kb on - strand, within DvMF_2559 at 3235.333 kb on - strand, within DvMF_2559 at 3235.346 kb on - strand, within DvMF_2559 at 3235.451 kb on - strand, within DvMF_2559 at 3235.710 kb on + strand, within DvMF_2559 at 3235.923 kb on + strand at 3235.960 kb on - strand at 3235.960 kb on - strand at 3235.970 kb on - strand at 3236.131 kb on - strand at 3236.190 kb on + strand, within DvMF_2560 at 3236.293 kb on - strand, within DvMF_2560 at 3236.329 kb on - strand, within DvMF_2560 at 3236.339 kb on - strand, within DvMF_2560 at 3236.429 kb on + strand, within DvMF_2560 at 3236.606 kb on - strand, within DvMF_2560 at 3236.709 kb on + strand, within DvMF_2560 at 3236.828 kb on + strand, within DvMF_2560 at 3236.901 kb on + strand, within DvMF_2560 at 3237.040 kb on + strand at 3237.047 kb on - strand at 3237.331 kb on + strand at 3237.590 kb on - strand, within DvMF_2562 at 3237.826 kb on - strand, within DvMF_2562 at 3238.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 3,235,216 - DvMF_2559 0.19 -0.6 3,235,266 - DvMF_2559 0.26 -0.8 3,235,325 + DvMF_2559 0.34 -0.1 3,235,325 + DvMF_2559 0.34 -0.4 3,235,333 - DvMF_2559 0.35 -0.4 3,235,333 - DvMF_2559 0.35 -0.1 3,235,346 - DvMF_2559 0.36 +0.4 3,235,451 - DvMF_2559 0.50 -1.0 3,235,710 + DvMF_2559 0.84 -1.9 3,235,923 + -0.8 3,235,960 - -1.2 3,235,960 - -1.0 3,235,970 - -0.6 3,236,131 - -1.4 3,236,190 + DvMF_2560 0.12 -1.1 3,236,293 - DvMF_2560 0.22 -0.7 3,236,329 - DvMF_2560 0.26 -1.4 3,236,339 - DvMF_2560 0.27 -0.1 3,236,429 + DvMF_2560 0.36 -0.9 3,236,606 - DvMF_2560 0.53 -1.5 3,236,709 + DvMF_2560 0.64 -1.0 3,236,828 + DvMF_2560 0.75 -0.8 3,236,901 + DvMF_2560 0.83 -1.1 3,237,040 + -1.6 3,237,047 - -1.7 3,237,331 + -1.0 3,237,590 - DvMF_2562 0.43 -1.2 3,237,826 - DvMF_2562 0.82 -0.7 3,238,020 - -0.8
Or see this region's nucleotide sequence