Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1846 and DvMF_1847 overlap by 8 nucleotides DvMF_1847 and DvMF_1848 are separated by 8 nucleotides DvMF_1848 and DvMF_1849 overlap by 4 nucleotides DvMF_1849 and DvMF_1850 are separated by 15 nucleotides
DvMF_1846: DvMF_1846 - UDP-N-acetylglucosamine 2-epimerase (RefSeq), at 2,257,737 to 2,258,963
_1846
DvMF_1847: DvMF_1847 - polysaccharide deacetylase (RefSeq), at 2,258,956 to 2,259,897
_1847
DvMF_1848: DvMF_1848 - hypothetical protein (RefSeq), at 2,259,906 to 2,261,060
_1848
DvMF_1849: DvMF_1849 - Radical SAM domain protein (RefSeq), at 2,261,057 to 2,261,989
_1849
DvMF_1850: DvMF_1850 - Capsule polysaccharide biosynthesis protein (RefSeq), at 2,262,005 to 2,263,510
_1850
Position (kb)
2259
2260
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1 at 2259.039 kb on - strand at 2259.046 kb on - strand at 2259.345 kb on + strand, within DvMF_1847 at 2259.348 kb on - strand, within DvMF_1847 at 2259.628 kb on - strand, within DvMF_1847 at 2259.714 kb on + strand, within DvMF_1847 at 2259.757 kb on + strand, within DvMF_1847 at 2259.803 kb on + strand at 2259.911 kb on + strand at 2259.947 kb on - strand at 2260.152 kb on + strand, within DvMF_1848 at 2260.256 kb on + strand, within DvMF_1848 at 2260.438 kb on - strand, within DvMF_1848 at 2260.593 kb on + strand, within DvMF_1848 at 2260.593 kb on + strand, within DvMF_1848 at 2260.616 kb on + strand, within DvMF_1848 at 2260.679 kb on - strand, within DvMF_1848 at 2260.681 kb on + strand, within DvMF_1848 at 2260.681 kb on + strand, within DvMF_1848 at 2260.703 kb on + strand, within DvMF_1848 at 2260.704 kb on - strand, within DvMF_1848 at 2260.890 kb on - strand, within DvMF_1848 at 2261.363 kb on + strand, within DvMF_1849 at 2261.363 kb on - strand, within DvMF_1849 at 2261.806 kb on + strand, within DvMF_1849 at 2261.927 kb on + strand at 2261.964 kb on + strand at 2261.972 kb on - strand at 2261.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 2,259,039 - -0.9 2,259,046 - -0.6 2,259,345 + DvMF_1847 0.41 -1.1 2,259,348 - DvMF_1847 0.42 -0.9 2,259,628 - DvMF_1847 0.71 -0.8 2,259,714 + DvMF_1847 0.80 -1.5 2,259,757 + DvMF_1847 0.85 -1.4 2,259,803 + -0.8 2,259,911 + -1.9 2,259,947 - -0.9 2,260,152 + DvMF_1848 0.21 -0.9 2,260,256 + DvMF_1848 0.30 -1.2 2,260,438 - DvMF_1848 0.46 -1.4 2,260,593 + DvMF_1848 0.59 -0.6 2,260,593 + DvMF_1848 0.59 -1.2 2,260,616 + DvMF_1848 0.61 -0.6 2,260,679 - DvMF_1848 0.67 -1.0 2,260,681 + DvMF_1848 0.67 -0.3 2,260,681 + DvMF_1848 0.67 -1.5 2,260,703 + DvMF_1848 0.69 -1.1 2,260,704 - DvMF_1848 0.69 -1.1 2,260,890 - DvMF_1848 0.85 -1.6 2,261,363 + DvMF_1849 0.33 -1.2 2,261,363 - DvMF_1849 0.33 -1.1 2,261,806 + DvMF_1849 0.80 -1.3 2,261,927 + -0.9 2,261,964 + -0.9 2,261,972 - -1.6 2,261,972 - -1.3
Or see this region's nucleotide sequence