Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1845 and DvMF_1846 overlap by 8 nucleotides DvMF_1846 and DvMF_1847 overlap by 8 nucleotides DvMF_1847 and DvMF_1848 are separated by 8 nucleotides
DvMF_1845: DvMF_1845 - N-acylneuraminate-9-phosphate synthase (RefSeq), at 2,256,710 to 2,257,744
_1845
DvMF_1846: DvMF_1846 - UDP-N-acetylglucosamine 2-epimerase (RefSeq), at 2,257,737 to 2,258,963
_1846
DvMF_1847: DvMF_1847 - polysaccharide deacetylase (RefSeq), at 2,258,956 to 2,259,897
_1847
DvMF_1848: DvMF_1848 - hypothetical protein (RefSeq), at 2,259,906 to 2,261,060
_1848
Position (kb)
2257
2258
2259 Strain fitness (log2 ratio)
-2
-1
0
1 at 2256.822 kb on - strand, within DvMF_1845 at 2256.953 kb on + strand, within DvMF_1845 at 2256.998 kb on + strand, within DvMF_1845 at 2257.050 kb on + strand, within DvMF_1845 at 2257.068 kb on - strand, within DvMF_1845 at 2257.190 kb on + strand, within DvMF_1845 at 2257.229 kb on + strand, within DvMF_1845 at 2257.463 kb on - strand, within DvMF_1845 at 2257.510 kb on - strand, within DvMF_1845 at 2257.510 kb on - strand, within DvMF_1845 at 2257.556 kb on + strand, within DvMF_1845 at 2257.598 kb on - strand, within DvMF_1845 at 2257.601 kb on + strand, within DvMF_1845 at 2257.601 kb on + strand, within DvMF_1845 at 2257.609 kb on - strand, within DvMF_1845 at 2257.790 kb on + strand at 2258.110 kb on + strand, within DvMF_1846 at 2258.252 kb on - strand, within DvMF_1846 at 2258.367 kb on - strand, within DvMF_1846 at 2258.375 kb on + strand, within DvMF_1846 at 2258.390 kb on + strand, within DvMF_1846 at 2258.429 kb on + strand, within DvMF_1846 at 2258.434 kb on + strand, within DvMF_1846 at 2258.437 kb on - strand, within DvMF_1846 at 2258.459 kb on - strand, within DvMF_1846 at 2258.575 kb on + strand, within DvMF_1846 at 2258.630 kb on - strand, within DvMF_1846 at 2258.817 kb on - strand, within DvMF_1846 at 2258.891 kb on + strand at 2259.039 kb on - strand at 2259.046 kb on - strand at 2259.345 kb on + strand, within DvMF_1847 at 2259.348 kb on - strand, within DvMF_1847 at 2259.628 kb on - strand, within DvMF_1847 at 2259.714 kb on + strand, within DvMF_1847 at 2259.757 kb on + strand, within DvMF_1847 at 2259.803 kb on + strand at 2259.911 kb on + strand at 2259.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 2,256,822 - DvMF_1845 0.11 -0.9 2,256,953 + DvMF_1845 0.23 -0.8 2,256,998 + DvMF_1845 0.28 -0.4 2,257,050 + DvMF_1845 0.33 -1.2 2,257,068 - DvMF_1845 0.35 -0.8 2,257,190 + DvMF_1845 0.46 -1.2 2,257,229 + DvMF_1845 0.50 -0.7 2,257,463 - DvMF_1845 0.73 -1.0 2,257,510 - DvMF_1845 0.77 -0.8 2,257,510 - DvMF_1845 0.77 -1.1 2,257,556 + DvMF_1845 0.82 -1.3 2,257,598 - DvMF_1845 0.86 -1.1 2,257,601 + DvMF_1845 0.86 -1.1 2,257,601 + DvMF_1845 0.86 -0.9 2,257,609 - DvMF_1845 0.87 -1.5 2,257,790 + -0.9 2,258,110 + DvMF_1846 0.30 -0.5 2,258,252 - DvMF_1846 0.42 -1.1 2,258,367 - DvMF_1846 0.51 -1.1 2,258,375 + DvMF_1846 0.52 -0.7 2,258,390 + DvMF_1846 0.53 -1.3 2,258,429 + DvMF_1846 0.56 -1.4 2,258,434 + DvMF_1846 0.57 -1.5 2,258,437 - DvMF_1846 0.57 -1.2 2,258,459 - DvMF_1846 0.59 -1.2 2,258,575 + DvMF_1846 0.68 -1.4 2,258,630 - DvMF_1846 0.73 -1.1 2,258,817 - DvMF_1846 0.88 -1.4 2,258,891 + -0.5 2,259,039 - -0.9 2,259,046 - -0.6 2,259,345 + DvMF_1847 0.41 -1.1 2,259,348 - DvMF_1847 0.42 -0.9 2,259,628 - DvMF_1847 0.71 -0.8 2,259,714 + DvMF_1847 0.80 -1.5 2,259,757 + DvMF_1847 0.85 -1.4 2,259,803 + -0.8 2,259,911 + -1.9 2,259,947 - -0.9
Or see this region's nucleotide sequence