Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1233 and DvMF_1234 are separated by 445 nucleotides DvMF_1234 and DvMF_1235 are separated by 55 nucleotides DvMF_1235 and DvMF_1236 are separated by 134 nucleotides
DvMF_1233: DvMF_1233 - formate dehydrogenase accessory protein (RefSeq), at 1,521,224 to 1,522,135
_1233
DvMF_1234: DvMF_1234 - methionine-R-sulfoxide reductase (RefSeq), at 1,522,581 to 1,523,453
_1234
DvMF_1235: DvMF_1235 - hypothetical protein (RefSeq), at 1,523,509 to 1,523,808
_1235
DvMF_1236: DvMF_1236 - hypothetical protein (RefSeq), at 1,523,943 to 1,524,692
_1236
Position (kb)
1522
1523
1524 Strain fitness (log2 ratio)
-1
0
1 at 1521.681 kb on - strand, within DvMF_1233 at 1521.751 kb on - strand, within DvMF_1233 at 1521.803 kb on - strand, within DvMF_1233 at 1521.846 kb on - strand, within DvMF_1233 at 1522.071 kb on + strand at 1522.242 kb on + strand at 1522.252 kb on - strand at 1522.366 kb on + strand at 1522.451 kb on + strand at 1522.478 kb on - strand at 1522.478 kb on - strand at 1523.062 kb on - strand, within DvMF_1234 at 1523.292 kb on - strand, within DvMF_1234 at 1523.483 kb on + strand at 1523.501 kb on - strand at 1523.527 kb on - strand at 1524.021 kb on - strand, within DvMF_1236 at 1524.035 kb on + strand, within DvMF_1236
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 1,521,681 - DvMF_1233 0.50 -0.5 1,521,751 - DvMF_1233 0.58 -0.5 1,521,803 - DvMF_1233 0.63 -0.4 1,521,846 - DvMF_1233 0.68 -0.1 1,522,071 + -1.2 1,522,242 + -0.7 1,522,252 - +0.6 1,522,366 + -0.4 1,522,451 + -0.4 1,522,478 - -0.6 1,522,478 - -0.8 1,523,062 - DvMF_1234 0.55 -0.8 1,523,292 - DvMF_1234 0.81 -0.2 1,523,483 + -0.5 1,523,501 - -0.7 1,523,527 - -1.0 1,524,021 - DvMF_1236 0.10 -0.5 1,524,035 + DvMF_1236 0.12 -0.6
Or see this region's nucleotide sequence