Experiment: MoYLS4 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0215 and DvMF_0216 are separated by 17 nucleotides DvMF_0216 and DvMF_0217 are separated by 289 nucleotides DvMF_0217 and DvMF_0218 are separated by 102 nucleotides
DvMF_0215: DvMF_0215 - two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq), at 242,020 to 243,606
_0215
DvMF_0216: DvMF_0216 - hypothetical protein (RefSeq), at 243,624 to 244,205
_0216
DvMF_0217: DvMF_0217 - Hpt protein (RefSeq), at 244,495 to 244,860
_0217
DvMF_0218: DvMF_0218 - hypothetical protein (RefSeq), at 244,963 to 245,226
_0218
Position (kb) 243
244
245 Strain fitness (log2 ratio) -2
-1
0
1 at 242.661 kb on + strand, within DvMF_0215 at 242.676 kb on + strand, within DvMF_0215 at 242.706 kb on + strand, within DvMF_0215 at 242.791 kb on + strand, within DvMF_0215 at 242.797 kb on - strand, within DvMF_0215 at 243.239 kb on - strand, within DvMF_0215 at 243.246 kb on - strand, within DvMF_0215 at 243.248 kb on + strand, within DvMF_0215 at 243.350 kb on - strand, within DvMF_0215 at 243.540 kb on + strand at 243.660 kb on - strand at 243.764 kb on - strand, within DvMF_0216 at 243.819 kb on + strand, within DvMF_0216 at 243.822 kb on - strand, within DvMF_0216 at 244.300 kb on - strand at 244.512 kb on - strand at 244.513 kb on - strand at 244.740 kb on + strand, within DvMF_0217 at 244.754 kb on - strand, within DvMF_0217 at 244.887 kb on + strand at 244.921 kb on + strand at 244.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nalidixic 0.0078125 mg/ml remove 242,661 + DvMF_0215 0.40 -0.6 242,676 + DvMF_0215 0.41 -0.4 242,706 + DvMF_0215 0.43 -1.1 242,791 + DvMF_0215 0.49 -0.9 242,797 - DvMF_0215 0.49 -0.9 243,239 - DvMF_0215 0.77 -0.2 243,246 - DvMF_0215 0.77 -1.1 243,248 + DvMF_0215 0.77 -0.5 243,350 - DvMF_0215 0.84 -1.9 243,540 + -1.0 243,660 - -0.9 243,764 - DvMF_0216 0.24 -2.1 243,819 + DvMF_0216 0.34 -0.3 243,822 - DvMF_0216 0.34 -0.9 244,300 - -1.5 244,512 - -1.3 244,513 - -0.6 244,740 + DvMF_0217 0.67 -1.6 244,754 - DvMF_0217 0.71 +0.2 244,887 + -0.7 244,921 + -0.9 244,939 - -1.6
Or see this region's nucleotide sequence