Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3727 and Shewana3_3728 are separated by 59 nucleotides Shewana3_3728 and Shewana3_3729 are separated by 6 nucleotides Shewana3_3729 and Shewana3_3730 are separated by 3 nucleotides Shewana3_3730 and Shewana3_3731 are separated by 44 nucleotides Shewana3_3731 and Shewana3_3732 overlap by 1 nucleotides
Shewana3_3727: Shewana3_3727 - 2-octaprenylphenol hydroxylase (RefSeq), at 4,469,495 to 4,471,144
_3727
Shewana3_3728: Shewana3_3728 - twin arginine-targeting protein translocase (RefSeq), at 4,471,204 to 4,471,461
_3728
Shewana3_3729: Shewana3_3729 - twin-arginine translocation protein, TatB subunit (RefSeq), at 4,471,468 to 4,471,911
_3729
Shewana3_3730: Shewana3_3730 - Sec-independent protein translocase TatC (RefSeq), at 4,471,915 to 4,472,664
_3730
Shewana3_3731: Shewana3_3731 - hypothetical protein (RefSeq), at 4,472,709 to 4,473,239
_3731
Shewana3_3732: Shewana3_3732 - Sec-independent protein translocase TatD (RefSeq), at 4,473,239 to 4,474,042
_3732
Position (kb) 4471
4472
4473 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 4471.130 kb on - strand at 4471.354 kb on + strand, within Shewana3_3728 at 4471.486 kb on - strand at 4471.958 kb on + strand at 4472.257 kb on - strand, within Shewana3_3730 at 4472.465 kb on - strand, within Shewana3_3730 at 4472.547 kb on + strand, within Shewana3_3730 at 4472.736 kb on + strand at 4472.736 kb on + strand at 4472.744 kb on - strand at 4472.756 kb on + strand at 4472.756 kb on + strand at 4472.764 kb on - strand, within Shewana3_3731 at 4472.892 kb on + strand, within Shewana3_3731 at 4472.939 kb on - strand, within Shewana3_3731 at 4472.959 kb on - strand, within Shewana3_3731 at 4472.969 kb on - strand, within Shewana3_3731 at 4473.208 kb on + strand at 4473.215 kb on + strand at 4473.465 kb on + strand, within Shewana3_3732 at 4473.533 kb on + strand, within Shewana3_3732 at 4473.583 kb on - strand, within Shewana3_3732
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,471,130 - -1.9 4,471,354 + Shewana3_3728 0.58 -2.5 4,471,486 - -3.2 4,471,958 + -3.8 4,472,257 - Shewana3_3730 0.46 -2.9 4,472,465 - Shewana3_3730 0.73 -4.8 4,472,547 + Shewana3_3730 0.84 +2.4 4,472,736 + -0.4 4,472,736 + +0.3 4,472,744 - +0.5 4,472,756 + +0.3 4,472,756 + -1.2 4,472,764 - Shewana3_3731 0.10 +0.6 4,472,892 + Shewana3_3731 0.34 +0.4 4,472,939 - Shewana3_3731 0.43 +0.3 4,472,959 - Shewana3_3731 0.47 +0.0 4,472,969 - Shewana3_3731 0.49 -0.0 4,473,208 + +0.0 4,473,215 + +0.6 4,473,465 + Shewana3_3732 0.28 +0.1 4,473,533 + Shewana3_3732 0.37 -0.1 4,473,583 - Shewana3_3732 0.43 -0.6
Or see this region's nucleotide sequence