Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2268 and Shewana3_2269 are separated by 468 nucleotides Shewana3_2269 and ilvH overlap by 1 nucleotides ilvH and Shewana3_2271 are separated by 134 nucleotides
Shewana3_2268: Shewana3_2268 - Bcr/CflA subfamily drug resistance transporter (RefSeq), at 2,731,359 to 2,732,582
_2268
Shewana3_2269: Shewana3_2269 - acetolactate synthase 3 catalytic subunit (RefSeq), at 2,733,051 to 2,734,769
_2269
Shewana3_2270: ilvH - acetolactate synthase 3 regulatory subunit (RefSeq), at 2,734,769 to 2,735,263
ilvH
Shewana3_2271: Shewana3_2271 - heat shock protein Hsp20 (RefSeq), at 2,735,398 to 2,735,841
_2271
Position (kb)
2733
2734
2735 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2732.404 kb on + strand, within Shewana3_2268 at 2733.138 kb on + strand at 2733.138 kb on + strand at 2733.146 kb on - strand at 2734.029 kb on + strand, within Shewana3_2269 at 2734.421 kb on + strand, within Shewana3_2269 at 2734.693 kb on - strand at 2734.695 kb on + strand at 2734.700 kb on + strand at 2735.123 kb on + strand, within ilvH at 2735.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,732,404 + Shewana3_2268 0.85 -0.3 2,733,138 + -3.1 2,733,138 + -4.5 2,733,146 - -4.4 2,734,029 + Shewana3_2269 0.57 -3.2 2,734,421 + Shewana3_2269 0.80 -5.0 2,734,693 - -4.3 2,734,695 + -3.7 2,734,700 + -3.6 2,735,123 + ilvH Shewana3_2270 0.72 -1.5 2,735,222 + -2.2
Or see this region's nucleotide sequence