Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1848 and Shewana3_1849 overlap by 19 nucleotides Shewana3_1849 and hisH are separated by 29 nucleotides hisH and Shewana3_1851 are separated by 145 nucleotides
Shewana3_1848: Shewana3_1848 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,190,056 to 2,190,829
_1848
Shewana3_1849: Shewana3_1849 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,190,811 to 2,191,548
_1849
Shewana3_1850: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,191,578 to 2,192,228
hisH
Shewana3_1851: Shewana3_1851 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,192,374 to 2,193,465
_1851
Position (kb)
2191
2192
2193 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2190.633 kb on + strand, within Shewana3_1848 at 2190.634 kb on + strand, within Shewana3_1848 at 2190.641 kb on - strand, within Shewana3_1848 at 2190.778 kb on - strand at 2190.826 kb on + strand at 2190.981 kb on + strand, within Shewana3_1849 at 2190.989 kb on - strand, within Shewana3_1849 at 2191.094 kb on - strand, within Shewana3_1849 at 2191.651 kb on + strand, within hisH at 2191.714 kb on - strand, within hisH at 2191.845 kb on + strand, within hisH at 2191.980 kb on - strand, within hisH at 2191.997 kb on + strand, within hisH at 2192.006 kb on - strand, within hisH at 2192.122 kb on + strand, within hisH at 2192.137 kb on - strand, within hisH at 2192.473 kb on - strand at 2192.490 kb on - strand, within Shewana3_1851 at 2192.499 kb on + strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.558 kb on - strand, within Shewana3_1851 at 2192.705 kb on + strand, within Shewana3_1851 at 2192.711 kb on - strand, within Shewana3_1851 at 2192.718 kb on - strand, within Shewana3_1851 at 2192.723 kb on - strand, within Shewana3_1851 at 2192.736 kb on - strand, within Shewana3_1851 at 2192.742 kb on + strand, within Shewana3_1851 at 2192.921 kb on - strand, within Shewana3_1851 at 2192.988 kb on - strand, within Shewana3_1851 at 2193.080 kb on - strand, within Shewana3_1851 at 2193.086 kb on - strand, within Shewana3_1851 at 2193.166 kb on + strand, within Shewana3_1851 at 2193.223 kb on - strand, within Shewana3_1851
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,190,633 + Shewana3_1848 0.75 -4.8 2,190,634 + Shewana3_1848 0.75 -4.2 2,190,641 - Shewana3_1848 0.76 -5.4 2,190,778 - -5.8 2,190,826 + -4.6 2,190,981 + Shewana3_1849 0.23 -4.3 2,190,989 - Shewana3_1849 0.24 -4.5 2,191,094 - Shewana3_1849 0.38 -6.5 2,191,651 + hisH Shewana3_1850 0.11 -3.4 2,191,714 - hisH Shewana3_1850 0.21 -5.9 2,191,845 + hisH Shewana3_1850 0.41 -6.6 2,191,980 - hisH Shewana3_1850 0.62 -4.2 2,191,997 + hisH Shewana3_1850 0.64 -4.5 2,192,006 - hisH Shewana3_1850 0.66 -6.8 2,192,122 + hisH Shewana3_1850 0.84 -3.2 2,192,137 - hisH Shewana3_1850 0.86 -6.4 2,192,473 - -6.7 2,192,490 - Shewana3_1851 0.11 -7.1 2,192,499 + Shewana3_1851 0.11 -6.7 2,192,507 - Shewana3_1851 0.12 -4.8 2,192,507 - Shewana3_1851 0.12 -4.7 2,192,507 - Shewana3_1851 0.12 -3.2 2,192,558 - Shewana3_1851 0.17 -6.1 2,192,705 + Shewana3_1851 0.30 -4.4 2,192,711 - Shewana3_1851 0.31 -6.0 2,192,718 - Shewana3_1851 0.32 -7.1 2,192,723 - Shewana3_1851 0.32 -5.5 2,192,736 - Shewana3_1851 0.33 -5.6 2,192,742 + Shewana3_1851 0.34 -3.7 2,192,921 - Shewana3_1851 0.50 -5.7 2,192,988 - Shewana3_1851 0.56 -5.9 2,193,080 - Shewana3_1851 0.65 -6.1 2,193,086 - Shewana3_1851 0.65 -4.6 2,193,166 + Shewana3_1851 0.73 -5.2 2,193,223 - Shewana3_1851 0.78 -2.8
Or see this region's nucleotide sequence