Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1109 and rumA are separated by 101 nucleotides rumA and Shewana3_1111 are separated by 189 nucleotides
Shewana3_1109: Shewana3_1109 - hybrid sensory histidine kinase BarA (RefSeq), at 1,302,416 to 1,305,223
_1109
Shewana3_1110: rumA - 23S rRNA 5-methyluridine methyltransferase (RefSeq), at 1,305,325 to 1,306,674
rumA
Shewana3_1111: Shewana3_1111 - (p)ppGpp synthetase I, SpoT/RelA (RefSeq), at 1,306,864 to 1,309,071
_1111
Position (kb) 1305
1306
1307 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1304.397 kb on - strand, within Shewana3_1109 at 1304.515 kb on + strand, within Shewana3_1109 at 1304.546 kb on - strand, within Shewana3_1109 at 1304.615 kb on + strand, within Shewana3_1109 at 1304.678 kb on + strand, within Shewana3_1109 at 1304.681 kb on - strand, within Shewana3_1109 at 1304.681 kb on - strand, within Shewana3_1109 at 1304.683 kb on - strand, within Shewana3_1109 at 1304.823 kb on - strand, within Shewana3_1109 at 1304.829 kb on - strand, within Shewana3_1109 at 1304.871 kb on + strand, within Shewana3_1109 at 1305.071 kb on + strand at 1305.192 kb on - strand at 1305.194 kb on + strand at 1305.218 kb on + strand at 1305.218 kb on + strand at 1305.218 kb on + strand at 1305.425 kb on - strand at 1305.528 kb on + strand, within rumA at 1305.551 kb on - strand, within rumA at 1305.595 kb on - strand, within rumA at 1305.841 kb on - strand, within rumA at 1305.843 kb on + strand, within rumA at 1305.844 kb on + strand, within rumA at 1305.913 kb on - strand, within rumA at 1306.132 kb on + strand, within rumA at 1306.145 kb on + strand, within rumA at 1306.145 kb on + strand, within rumA at 1306.146 kb on - strand, within rumA at 1306.153 kb on - strand, within rumA at 1306.223 kb on + strand, within rumA at 1306.385 kb on + strand, within rumA at 1306.496 kb on + strand, within rumA at 1306.496 kb on + strand, within rumA at 1306.573 kb on - strand at 1306.785 kb on - strand at 1306.829 kb on + strand at 1306.970 kb on + strand at 1307.009 kb on - strand at 1307.062 kb on + strand at 1307.595 kb on - strand, within Shewana3_1111 at 1307.604 kb on + strand, within Shewana3_1111 at 1307.605 kb on + strand, within Shewana3_1111 at 1307.605 kb on + strand, within Shewana3_1111 at 1307.669 kb on - strand, within Shewana3_1111
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 1,304,397 - Shewana3_1109 0.71 +0.0 1,304,515 + Shewana3_1109 0.75 +0.6 1,304,546 - Shewana3_1109 0.76 -0.1 1,304,615 + Shewana3_1109 0.78 +0.3 1,304,678 + Shewana3_1109 0.81 -0.4 1,304,681 - Shewana3_1109 0.81 +0.3 1,304,681 - Shewana3_1109 0.81 +0.3 1,304,683 - Shewana3_1109 0.81 +0.4 1,304,823 - Shewana3_1109 0.86 +0.5 1,304,829 - Shewana3_1109 0.86 -0.2 1,304,871 + Shewana3_1109 0.87 +0.5 1,305,071 + -0.3 1,305,192 - +0.4 1,305,194 + +0.4 1,305,218 + +0.5 1,305,218 + +0.5 1,305,218 + +0.9 1,305,425 - -2.0 1,305,528 + rumA Shewana3_1110 0.15 +1.0 1,305,551 - rumA Shewana3_1110 0.17 +1.2 1,305,595 - rumA Shewana3_1110 0.20 -1.3 1,305,841 - rumA Shewana3_1110 0.38 +0.9 1,305,843 + rumA Shewana3_1110 0.38 +0.9 1,305,844 + rumA Shewana3_1110 0.38 +0.9 1,305,913 - rumA Shewana3_1110 0.44 -0.1 1,306,132 + rumA Shewana3_1110 0.60 -0.9 1,306,145 + rumA Shewana3_1110 0.61 -0.2 1,306,145 + rumA Shewana3_1110 0.61 -0.4 1,306,146 - rumA Shewana3_1110 0.61 -0.9 1,306,153 - rumA Shewana3_1110 0.61 -0.4 1,306,223 + rumA Shewana3_1110 0.67 -0.1 1,306,385 + rumA Shewana3_1110 0.79 +0.1 1,306,496 + rumA Shewana3_1110 0.87 -0.7 1,306,496 + rumA Shewana3_1110 0.87 -0.4 1,306,573 - -0.1 1,306,785 - -0.8 1,306,829 + -0.3 1,306,970 + -5.3 1,307,009 - -1.0 1,307,062 + -5.2 1,307,595 - Shewana3_1111 0.33 -6.7 1,307,604 + Shewana3_1111 0.34 -2.0 1,307,605 + Shewana3_1111 0.34 -5.3 1,307,605 + Shewana3_1111 0.34 -3.2 1,307,669 - Shewana3_1111 0.36 -3.6
Or see this region's nucleotide sequence