Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0681 and Shewana3_0682 are separated by 5 nucleotides Shewana3_0682 and Shewana3_0683 are separated by 16 nucleotides Shewana3_0683 and Shewana3_0684 are separated by 12 nucleotides Shewana3_0684 and Shewana3_0685 are separated by 99 nucleotides Shewana3_0685 and Shewana3_0686 are separated by 80 nucleotides
Shewana3_0681: Shewana3_0681 - toluene tolerance family protein (RefSeq), at 799,599 to 800,252
_0681
Shewana3_0682: Shewana3_0682 - SpoIIAA family protein (RefSeq), at 800,258 to 800,542
_0682
Shewana3_0683: Shewana3_0683 - BolA family protein (RefSeq), at 800,559 to 800,810
_0683
Shewana3_0684: Shewana3_0684 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (RefSeq), at 800,823 to 802,082
_0684
Shewana3_0685: Shewana3_0685 - TetR family transcriptional regulator (RefSeq), at 802,182 to 802,760
_0685
Shewana3_0686: Shewana3_0686 - hypothetical protein (RefSeq), at 802,841 to 803,701
_0686
Position (kb) 800
801
802
803 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 799.845 kb on + strand, within Shewana3_0681 at 799.915 kb on + strand, within Shewana3_0681 at 800.089 kb on + strand, within Shewana3_0681 at 800.134 kb on + strand, within Shewana3_0681 at 800.234 kb on + strand at 800.234 kb on + strand at 800.461 kb on + strand, within Shewana3_0682 at 800.618 kb on + strand, within Shewana3_0683 at 802.234 kb on + strand at 802.235 kb on + strand at 802.260 kb on + strand, within Shewana3_0685 at 802.260 kb on + strand, within Shewana3_0685 at 802.268 kb on - strand, within Shewana3_0685 at 802.268 kb on - strand, within Shewana3_0685 at 802.268 kb on - strand, within Shewana3_0685 at 802.313 kb on - strand, within Shewana3_0685 at 802.416 kb on - strand, within Shewana3_0685 at 802.682 kb on + strand, within Shewana3_0685 at 802.921 kb on - strand at 803.013 kb on + strand, within Shewana3_0686 at 803.038 kb on + strand, within Shewana3_0686
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 799,845 + Shewana3_0681 0.38 -5.7 799,915 + Shewana3_0681 0.48 -2.9 800,089 + Shewana3_0681 0.75 -1.5 800,134 + Shewana3_0681 0.82 -2.6 800,234 + -2.4 800,234 + -1.8 800,461 + Shewana3_0682 0.71 -4.0 800,618 + Shewana3_0683 0.23 +0.2 802,234 + -0.9 802,235 + +0.0 802,260 + Shewana3_0685 0.13 +0.3 802,260 + Shewana3_0685 0.13 -0.5 802,268 - Shewana3_0685 0.15 -0.6 802,268 - Shewana3_0685 0.15 +0.1 802,268 - Shewana3_0685 0.15 +0.9 802,313 - Shewana3_0685 0.23 -0.5 802,416 - Shewana3_0685 0.40 +0.4 802,682 + Shewana3_0685 0.86 +0.1 802,921 - -0.2 803,013 + Shewana3_0686 0.20 +0.3 803,038 + Shewana3_0686 0.23 +0.1
Or see this region's nucleotide sequence