Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0227 and Shewana3_0228 overlap by 4 nucleotides Shewana3_0228 and Shewana3_0229 overlap by 1 nucleotides Shewana3_0229 and Shewana3_0230 are separated by 1 nucleotides Shewana3_0230 and Shewana3_0231 are separated by 11 nucleotides Shewana3_0231 and Shewana3_0232 are separated by 325 nucleotides
Shewana3_0227: Shewana3_0227 - cytochrome c-type biogenesis protein CcmE (RefSeq), at 258,101 to 258,586
_0227
Shewana3_0228: Shewana3_0228 - Heme exporter protein D (CcmD) (RefSeq), at 258,583 to 258,783
_0228
Shewana3_0229: Shewana3_0229 - heme exporter protein CcmC (RefSeq), at 258,783 to 259,529
_0229
Shewana3_0230: Shewana3_0230 - heme exporter protein CcmB (RefSeq), at 259,531 to 260,217
_0230
Shewana3_0231: Shewana3_0231 - cytochrome c biogenesis protein CcmA (RefSeq), at 260,229 to 260,879
_0231
Shewana3_0232: Shewana3_0232 - cytochrome c, class I (RefSeq), at 261,205 to 261,504
_0232
Position (kb) 259
260
261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 258.654 kb on - strand, within Shewana3_0228 at 258.737 kb on - strand, within Shewana3_0228 at 258.807 kb on - strand at 259.003 kb on - strand, within Shewana3_0229 at 259.198 kb on + strand, within Shewana3_0229 at 259.512 kb on - strand at 259.572 kb on + strand at 259.734 kb on + strand, within Shewana3_0230 at 259.742 kb on + strand, within Shewana3_0230 at 259.789 kb on - strand, within Shewana3_0230 at 259.799 kb on + strand, within Shewana3_0230 at 259.932 kb on + strand, within Shewana3_0230 at 259.937 kb on + strand, within Shewana3_0230 at 259.940 kb on - strand, within Shewana3_0230 at 260.172 kb on + strand at 260.201 kb on - strand at 260.226 kb on + strand at 260.234 kb on - strand at 260.307 kb on - strand, within Shewana3_0231 at 260.451 kb on + strand, within Shewana3_0231 at 260.537 kb on + strand, within Shewana3_0231 at 260.593 kb on + strand, within Shewana3_0231 at 260.696 kb on + strand, within Shewana3_0231 at 260.697 kb on - strand, within Shewana3_0231 at 260.697 kb on - strand, within Shewana3_0231 at 260.700 kb on + strand, within Shewana3_0231 at 260.704 kb on - strand, within Shewana3_0231 at 260.708 kb on - strand, within Shewana3_0231 at 260.708 kb on - strand, within Shewana3_0231 at 260.835 kb on - strand at 260.846 kb on + strand at 260.955 kb on - strand at 260.955 kb on - strand at 260.993 kb on + strand at 261.129 kb on + strand at 261.130 kb on + strand at 261.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 258,654 - Shewana3_0228 0.35 -1.9 258,737 - Shewana3_0228 0.77 -2.2 258,807 - +0.7 259,003 - Shewana3_0229 0.29 +1.5 259,198 + Shewana3_0229 0.56 -2.6 259,512 - -2.1 259,572 + -4.4 259,734 + Shewana3_0230 0.30 -2.8 259,742 + Shewana3_0230 0.31 -3.4 259,789 - Shewana3_0230 0.38 -0.1 259,799 + Shewana3_0230 0.39 -1.7 259,932 + Shewana3_0230 0.58 -1.7 259,937 + Shewana3_0230 0.59 -0.8 259,940 - Shewana3_0230 0.60 -2.6 260,172 + +1.5 260,201 - -4.1 260,226 + -3.1 260,234 - -0.2 260,307 - Shewana3_0231 0.12 -2.6 260,451 + Shewana3_0231 0.34 -2.9 260,537 + Shewana3_0231 0.47 -1.3 260,593 + Shewana3_0231 0.56 -3.0 260,696 + Shewana3_0231 0.72 -1.6 260,697 - Shewana3_0231 0.72 -0.8 260,697 - Shewana3_0231 0.72 -2.7 260,700 + Shewana3_0231 0.72 -3.2 260,704 - Shewana3_0231 0.73 -1.6 260,708 - Shewana3_0231 0.74 -2.8 260,708 - Shewana3_0231 0.74 -2.8 260,835 - +0.3 260,846 + -0.2 260,955 - +0.1 260,955 - +0.3 260,993 + +0.2 261,129 + -1.2 261,130 + -0.4 261,147 - +0.2
Or see this region's nucleotide sequence