Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4282 and Shewana3_4283 are separated by 9 nucleotides Shewana3_4283 and Shewana3_4284 are separated by 495 nucleotides Shewana3_4284 and Shewana3_4285 are separated by 274 nucleotides Shewana3_4285 and Shewana3_4286 are separated by 136 nucleotides
Shewana3_4282: Shewana3_4282 - IS4 family transposase (RefSeq), at 166,549 to 167,286
_4282
Shewana3_4283: Shewana3_4283 - transposase (RefSeq), at 167,296 to 167,466
_4283
Shewana3_4284: Shewana3_4284 - integrase catalytic subunit (RefSeq), at 167,962 to 168,783
_4284
Shewana3_4285: Shewana3_4285 - hypothetical protein (RefSeq), at 169,058 to 169,498
_4285
Shewana3_4286: Shewana3_4286 - hypothetical protein (RefSeq), at 169,635 to 170,150
_4286
Position (kb) 167
168
169 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 167.316 kb on + strand, within Shewana3_4283 at 167.324 kb on - strand, within Shewana3_4283 at 167.571 kb on - strand at 167.575 kb on + strand at 167.598 kb on + strand at 167.650 kb on - strand at 167.662 kb on + strand at 167.715 kb on - strand at 167.794 kb on - strand at 167.796 kb on - strand at 167.796 kb on - strand at 167.796 kb on - strand at 167.944 kb on + strand at 168.069 kb on + strand, within Shewana3_4284 at 168.147 kb on + strand, within Shewana3_4284 at 168.155 kb on + strand, within Shewana3_4284 at 168.155 kb on - strand, within Shewana3_4284 at 168.163 kb on - strand, within Shewana3_4284 at 168.190 kb on - strand, within Shewana3_4284 at 168.252 kb on - strand, within Shewana3_4284 at 168.276 kb on + strand, within Shewana3_4284 at 168.308 kb on + strand, within Shewana3_4284 at 168.308 kb on - strand, within Shewana3_4284 at 168.396 kb on - strand, within Shewana3_4284 at 168.500 kb on - strand, within Shewana3_4284 at 168.557 kb on + strand, within Shewana3_4284 at 168.565 kb on - strand, within Shewana3_4284 at 168.644 kb on - strand, within Shewana3_4284 at 168.693 kb on - strand, within Shewana3_4284 at 168.693 kb on - strand, within Shewana3_4284 at 168.695 kb on + strand, within Shewana3_4284 at 168.810 kb on - strand at 168.887 kb on + strand at 168.913 kb on + strand at 168.913 kb on + strand at 168.966 kb on + strand at 168.973 kb on + strand at 168.973 kb on + strand at 168.973 kb on + strand at 168.981 kb on - strand at 168.981 kb on - strand at 169.098 kb on + strand at 169.276 kb on + strand, within Shewana3_4285 at 169.644 kb on + strand at 169.752 kb on - strand, within Shewana3_4286
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 167,316 + Shewana3_4283 0.12 +1.2 167,324 - Shewana3_4283 0.16 -0.3 167,571 - -0.4 167,575 + -1.0 167,598 + -0.1 167,650 - -0.4 167,662 + +0.0 167,715 - +1.0 167,794 - +0.1 167,796 - -0.1 167,796 - -0.1 167,796 - -0.2 167,944 + +0.5 168,069 + Shewana3_4284 0.13 +0.7 168,147 + Shewana3_4284 0.23 -0.4 168,155 + Shewana3_4284 0.23 +0.6 168,155 - Shewana3_4284 0.23 -4.3 168,163 - Shewana3_4284 0.24 +0.6 168,190 - Shewana3_4284 0.28 +0.4 168,252 - Shewana3_4284 0.35 +1.1 168,276 + Shewana3_4284 0.38 +1.0 168,308 + Shewana3_4284 0.42 +2.8 168,308 - Shewana3_4284 0.42 -1.3 168,396 - Shewana3_4284 0.53 +0.7 168,500 - Shewana3_4284 0.65 +1.2 168,557 + Shewana3_4284 0.72 +0.0 168,565 - Shewana3_4284 0.73 +0.1 168,644 - Shewana3_4284 0.83 +0.6 168,693 - Shewana3_4284 0.89 +0.8 168,693 - Shewana3_4284 0.89 -0.0 168,695 + Shewana3_4284 0.89 +0.4 168,810 - +0.3 168,887 + -0.1 168,913 + -1.1 168,913 + +0.3 168,966 + -0.4 168,973 + +1.1 168,973 + -0.0 168,973 + +0.2 168,981 - +0.9 168,981 - +0.2 169,098 + +0.5 169,276 + Shewana3_4285 0.49 +2.4 169,644 + -0.9 169,752 - Shewana3_4286 0.23 +1.4
Or see this region's nucleotide sequence