Experiment: MoYLS4 with G418 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2185 and DvMF_2186 are separated by 245 nucleotides DvMF_2186 and DvMF_2187 overlap by 4 nucleotides
DvMF_2185: DvMF_2185 - ThiJ/PfpI domain protein (RefSeq), at 2,727,957 to 2,728,697
_2185
DvMF_2186: DvMF_2186 - dual specificity protein phosphatase (RefSeq), at 2,728,943 to 2,730,445
_2186
DvMF_2187: DvMF_2187 - pyruvate phosphate dikinase PEP/pyruvate-binding (RefSeq), at 2,730,442 to 2,733,027
_2187
Position (kb)
2728
2729
2730
2731 Strain fitness (log2 ratio)
-1
0
1 at 2728.013 kb on - strand at 2728.118 kb on + strand, within DvMF_2185 at 2728.148 kb on - strand, within DvMF_2185 at 2728.153 kb on - strand, within DvMF_2185 at 2728.705 kb on - strand at 2728.876 kb on + strand at 2728.889 kb on + strand at 2728.920 kb on - strand at 2729.109 kb on - strand, within DvMF_2186 at 2729.163 kb on - strand, within DvMF_2186 at 2729.169 kb on + strand, within DvMF_2186 at 2729.266 kb on - strand, within DvMF_2186 at 2729.350 kb on + strand, within DvMF_2186 at 2729.570 kb on - strand, within DvMF_2186 at 2729.606 kb on - strand, within DvMF_2186 at 2729.711 kb on - strand, within DvMF_2186 at 2729.856 kb on - strand, within DvMF_2186 at 2729.953 kb on + strand, within DvMF_2186 at 2729.957 kb on - strand, within DvMF_2186 at 2730.065 kb on + strand, within DvMF_2186 at 2730.073 kb on - strand, within DvMF_2186 at 2730.192 kb on - strand, within DvMF_2186 at 2730.219 kb on + strand, within DvMF_2186 at 2730.307 kb on + strand at 2730.340 kb on + strand at 2730.690 kb on + strand at 2730.751 kb on + strand, within DvMF_2187 at 2730.759 kb on - strand, within DvMF_2187 at 2731.022 kb on + strand, within DvMF_2187 at 2731.079 kb on - strand, within DvMF_2187 at 2731.251 kb on - strand, within DvMF_2187 at 2731.383 kb on + strand, within DvMF_2187 at 2731.383 kb on + strand, within DvMF_2187
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with G418 0.125 mg/ml remove 2,728,013 - -0.6 2,728,118 + DvMF_2185 0.22 -0.1 2,728,148 - DvMF_2185 0.26 -0.5 2,728,153 - DvMF_2185 0.26 -0.1 2,728,705 - -0.4 2,728,876 + +0.1 2,728,889 + -0.5 2,728,920 - +0.2 2,729,109 - DvMF_2186 0.11 -0.3 2,729,163 - DvMF_2186 0.15 +0.7 2,729,169 + DvMF_2186 0.15 -0.8 2,729,266 - DvMF_2186 0.21 -0.3 2,729,350 + DvMF_2186 0.27 -0.7 2,729,570 - DvMF_2186 0.42 -0.6 2,729,606 - DvMF_2186 0.44 -0.4 2,729,711 - DvMF_2186 0.51 -0.2 2,729,856 - DvMF_2186 0.61 -0.2 2,729,953 + DvMF_2186 0.67 -0.7 2,729,957 - DvMF_2186 0.67 -0.2 2,730,065 + DvMF_2186 0.75 -0.3 2,730,073 - DvMF_2186 0.75 -0.2 2,730,192 - DvMF_2186 0.83 +0.1 2,730,219 + DvMF_2186 0.85 -0.5 2,730,307 + -0.5 2,730,340 + -0.4 2,730,690 + -0.5 2,730,751 + DvMF_2187 0.12 -0.5 2,730,759 - DvMF_2187 0.12 -0.2 2,731,022 + DvMF_2187 0.22 -0.3 2,731,079 - DvMF_2187 0.25 +0.3 2,731,251 - DvMF_2187 0.31 -0.3 2,731,383 + DvMF_2187 0.36 -0.0 2,731,383 + DvMF_2187 0.36 -0.0
Or see this region's nucleotide sequence