Experiment: MoYLS4 with G418 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisG and hisI overlap by 1 nucleotides hisI and DvMF_1965 are separated by 206 nucleotides DvMF_1965 and DvMF_1966 are separated by 238 nucleotides
DvMF_1963: hisG - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 2,426,158 to 2,427,039
hisG
DvMF_1964: hisI - phosphoribosyl-AMP cyclohydrolase (RefSeq), at 2,427,039 to 2,427,440
hisI
DvMF_1965: DvMF_1965 - class II aldolase/adducin family protein (RefSeq), at 2,427,647 to 2,428,804
_1965
DvMF_1966: DvMF_1966 - thiamine-monophosphate kinase (RefSeq), at 2,429,043 to 2,430,140
_1966
Position (kb)
2427
2428
2429 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2426.657 kb on - strand, within hisG at 2426.861 kb on - strand, within hisG at 2427.053 kb on + strand at 2427.131 kb on + strand, within hisI at 2427.303 kb on + strand, within hisI at 2427.436 kb on + strand at 2427.553 kb on + strand at 2427.584 kb on + strand at 2427.592 kb on - strand at 2427.592 kb on - strand at 2427.653 kb on - strand at 2427.658 kb on - strand at 2427.685 kb on + strand at 2427.712 kb on - strand at 2427.729 kb on - strand at 2427.750 kb on + strand at 2427.758 kb on - strand at 2427.763 kb on - strand, within DvMF_1965 at 2427.821 kb on - strand, within DvMF_1965 at 2428.037 kb on + strand, within DvMF_1965 at 2428.045 kb on - strand, within DvMF_1965 at 2428.092 kb on - strand, within DvMF_1965 at 2428.110 kb on + strand, within DvMF_1965 at 2428.115 kb on + strand, within DvMF_1965 at 2428.127 kb on + strand, within DvMF_1965 at 2428.223 kb on + strand, within DvMF_1965 at 2428.360 kb on + strand, within DvMF_1965 at 2428.527 kb on + strand, within DvMF_1965 at 2428.569 kb on - strand, within DvMF_1965 at 2429.081 kb on + strand at 2429.081 kb on + strand at 2429.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with G418 0.125 mg/ml remove 2,426,657 - hisG DvMF_1963 0.57 -0.5 2,426,861 - hisG DvMF_1963 0.80 -0.3 2,427,053 + -0.2 2,427,131 + hisI DvMF_1964 0.23 -0.0 2,427,303 + hisI DvMF_1964 0.66 -1.2 2,427,436 + -0.3 2,427,553 + -0.3 2,427,584 + -0.8 2,427,592 - -0.3 2,427,592 - -0.4 2,427,653 - -0.2 2,427,658 - -1.4 2,427,685 + +0.0 2,427,712 - -0.0 2,427,729 - -0.1 2,427,750 + -0.1 2,427,758 - -0.4 2,427,763 - DvMF_1965 0.10 -0.6 2,427,821 - DvMF_1965 0.15 -0.9 2,428,037 + DvMF_1965 0.34 -1.1 2,428,045 - DvMF_1965 0.34 -0.2 2,428,092 - DvMF_1965 0.38 -0.4 2,428,110 + DvMF_1965 0.40 -0.6 2,428,115 + DvMF_1965 0.40 -0.3 2,428,127 + DvMF_1965 0.41 -0.8 2,428,223 + DvMF_1965 0.50 -0.5 2,428,360 + DvMF_1965 0.62 -0.3 2,428,527 + DvMF_1965 0.76 -0.0 2,428,569 - DvMF_1965 0.80 -0.3 2,429,081 + -3.1 2,429,081 + +0.3 2,429,089 - -0.3
Or see this region's nucleotide sequence