Experiment: Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3482 and dut overlap by 4 nucleotides dut and slmA are separated by 130 nucleotides slmA and Shew_3485 are separated by 149 nucleotides
Shew_3482: Shew_3482 - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (RefSeq), at 4,167,530 to 4,168,726
_3482
Shew_3483: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (RefSeq), at 4,168,723 to 4,169,181
dut
Shew_3484: slmA - nucleoid occlusion protein (RefSeq), at 4,169,312 to 4,169,905
slmA
Shew_3485: Shew_3485 - peptidase S9 prolyl oligopeptidase (RefSeq), at 4,170,055 to 4,172,019
_3485
Position (kb)
4169
4170 Strain fitness (log2 ratio)
-2
-1
0
1 at 4168.811 kb on + strand, within dut at 4168.838 kb on + strand, within dut at 4169.174 kb on - strand at 4169.256 kb on + strand at 4169.759 kb on - strand, within slmA at 4169.867 kb on - strand at 4170.290 kb on + strand, within Shew_3485 at 4170.378 kb on + strand, within Shew_3485 at 4170.396 kb on - strand, within Shew_3485 at 4170.421 kb on - strand, within Shew_3485 at 4170.603 kb on + strand, within Shew_3485 at 4170.611 kb on - strand, within Shew_3485 at 4170.754 kb on + strand, within Shew_3485 at 4170.773 kb on - strand, within Shew_3485 at 4170.826 kb on + strand, within Shew_3485 at 4170.834 kb on - strand, within Shew_3485 at 4170.867 kb on + strand, within Shew_3485
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.08 mM remove 4,168,811 + dut Shew_3483 0.19 -1.5 4,168,838 + dut Shew_3483 0.25 -2.3 4,169,174 - -2.1 4,169,256 + -0.4 4,169,759 - slmA Shew_3484 0.75 -1.2 4,169,867 - -1.9 4,170,290 + Shew_3485 0.12 -0.3 4,170,378 + Shew_3485 0.16 -0.3 4,170,396 - Shew_3485 0.17 +0.0 4,170,421 - Shew_3485 0.19 -0.4 4,170,603 + Shew_3485 0.28 -0.2 4,170,611 - Shew_3485 0.28 -0.2 4,170,754 + Shew_3485 0.36 +0.1 4,170,773 - Shew_3485 0.37 +0.1 4,170,826 + Shew_3485 0.39 +0.2 4,170,834 - Shew_3485 0.40 +1.0 4,170,867 + Shew_3485 0.41 +0.2
Or see this region's nucleotide sequence