Strain Fitness in Shewanella loihica PV-4 around Shew_2202

Experiment: Cobalt chloride 0.08 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_2200 and hisH overlap by 4 nucleotideshisH and Shew_2202 overlap by 4 nucleotidesShew_2202 and Shew_2203 overlap by 19 nucleotidesShew_2203 and Shew_2204 are separated by 28 nucleotides Shew_2200: Shew_2200 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,560,961 to 2,562,031 _2200 Shew_2201: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,562,028 to 2,562,702 hisH Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436 _2202 Shew_2203: Shew_2203 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,563,418 to 2,564,191 _2203 Shew_2204: Shew_2204 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,564,220 to 2,564,840 _2204 Position (kb) 2562 2563 2564Strain fitness (log2 ratio) -1 0 1at 2561.750 kb on + strand, within Shew_2200at 2561.758 kb on - strand, within Shew_2200at 2562.347 kb on + strand, within hisHat 2562.722 kb on + strandat 2562.800 kb on + strand, within Shew_2202at 2563.591 kb on - strand, within Shew_2203at 2563.902 kb on + strand, within Shew_2203at 2563.973 kb on - strand, within Shew_2203at 2564.354 kb on - strand, within Shew_2204

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.08 mM
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2,561,750 + Shew_2200 0.74 -0.2
2,561,758 - Shew_2200 0.74 -0.2
2,562,347 + hisH Shew_2201 0.47 -0.1
2,562,722 + +0.1
2,562,800 + Shew_2202 0.14 +1.3
2,563,591 - Shew_2203 0.22 +0.2
2,563,902 + Shew_2203 0.63 -0.0
2,563,973 - Shew_2203 0.72 -0.1
2,564,354 - Shew_2204 0.22 -0.2

Or see this region's nucleotide sequence