Strain Fitness in Shewanella loihica PV-4 around Shew_2162

Experiment: Cobalt chloride 0.08 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_2161 and cpdB are separated by 305 nucleotidescpdB and Shew_2163 are separated by 123 nucleotidesShew_2163 and Shew_2164 are separated by 112 nucleotides Shew_2161: Shew_2161 - peptidyl-dipeptidase Dcp (RefSeq), at 2,508,721 to 2,510,901 _2161 Shew_2162: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 2,511,207 to 2,513,291 cpdB Shew_2163: Shew_2163 - hypothetical protein (RefSeq), at 2,513,415 to 2,514,017 _2163 Shew_2164: Shew_2164 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,514,130 to 2,515,920 _2164 Position (kb) 2511 2512 2513 2514Strain fitness (log2 ratio) -1 0 1at 2510.258 kb on - strand, within Shew_2161at 2510.879 kb on + strandat 2511.101 kb on + strandat 2511.109 kb on - strandat 2511.952 kb on - strand, within cpdBat 2512.021 kb on - strand, within cpdBat 2512.162 kb on + strand, within cpdBat 2512.322 kb on - strand, within cpdBat 2512.343 kb on + strand, within cpdBat 2512.494 kb on + strand, within cpdBat 2512.862 kb on + strand, within cpdBat 2513.165 kb on - strandat 2513.952 kb on + strand, within Shew_2163at 2514.210 kb on - strandat 2514.291 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.08 mM
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2,510,258 - Shew_2161 0.70 +0.3
2,510,879 + +0.4
2,511,101 + -0.5
2,511,109 - +0.0
2,511,952 - cpdB Shew_2162 0.36 +1.0
2,512,021 - cpdB Shew_2162 0.39 +0.6
2,512,162 + cpdB Shew_2162 0.46 +1.7
2,512,322 - cpdB Shew_2162 0.53 -0.3
2,512,343 + cpdB Shew_2162 0.54 +0.7
2,512,494 + cpdB Shew_2162 0.62 +0.4
2,512,862 + cpdB Shew_2162 0.79 -0.3
2,513,165 - -0.0
2,513,952 + Shew_2163 0.89 -1.0
2,514,210 - +1.4
2,514,291 + -0.7

Or see this region's nucleotide sequence