Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3918 and Dshi_3919 are separated by 315 nucleotides Dshi_3919 and Dshi_3920 are separated by 132 nucleotides Dshi_3920 and Dshi_3921 are separated by 26 nucleotides Dshi_3921 and Dshi_3922 are separated by 432 nucleotides
Dshi_3918: Dshi_3918 - hypothetical protein (RefSeq), at 145,009 to 145,482
_3918
Dshi_3919: Dshi_3919 - transposase IS3/IS911 family protein (RefSeq), at 145,798 to 146,166
_3919
Dshi_3920: Dshi_3920 - integrase, catalytic region (RefSeq), at 146,299 to 146,496
_3920
Dshi_3921: Dshi_3921 - Integrase catalytic region (RefSeq), at 146,523 to 146,837
_3921
Dshi_3922: Dshi_3922 - transport protein, putative (RefSeq), at 147,270 to 148,109
_3922
Position (kb)
146
147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 145.324 kb on - strand, within Dshi_3918 at 145.356 kb on + strand, within Dshi_3918 at 145.356 kb on - strand, within Dshi_3918 at 145.387 kb on + strand, within Dshi_3918 at 145.417 kb on - strand, within Dshi_3918 at 145.429 kb on + strand, within Dshi_3918 at 145.431 kb on + strand, within Dshi_3918 at 145.431 kb on + strand, within Dshi_3918 at 145.436 kb on + strand at 145.437 kb on - strand at 145.439 kb on - strand at 145.439 kb on - strand at 145.439 kb on - strand at 145.440 kb on - strand at 145.441 kb on + strand at 145.452 kb on + strand at 145.460 kb on - strand at 145.460 kb on - strand at 145.474 kb on - strand at 145.476 kb on + strand at 145.531 kb on - strand at 145.547 kb on - strand at 145.594 kb on + strand at 145.594 kb on + strand at 145.594 kb on + strand at 145.602 kb on - strand at 145.635 kb on - strand at 145.657 kb on - strand at 145.674 kb on + strand at 145.753 kb on - strand at 145.771 kb on - strand at 145.824 kb on + strand at 145.825 kb on - strand at 145.851 kb on - strand, within Dshi_3919 at 145.884 kb on - strand, within Dshi_3919 at 145.895 kb on - strand, within Dshi_3919 at 145.922 kb on + strand, within Dshi_3919 at 145.965 kb on - strand, within Dshi_3919 at 145.969 kb on - strand, within Dshi_3919 at 145.971 kb on - strand, within Dshi_3919 at 145.972 kb on + strand, within Dshi_3919 at 145.972 kb on + strand, within Dshi_3919 at 145.973 kb on - strand, within Dshi_3919 at 145.996 kb on + strand, within Dshi_3919 at 146.001 kb on + strand, within Dshi_3919 at 146.099 kb on + strand, within Dshi_3919 at 146.121 kb on + strand, within Dshi_3919 at 146.199 kb on + strand at 146.227 kb on + strand at 146.235 kb on + strand at 146.237 kb on - strand at 146.242 kb on - strand at 146.272 kb on + strand at 146.276 kb on + strand at 146.280 kb on - strand at 146.287 kb on + strand at 146.312 kb on + strand at 146.333 kb on + strand, within Dshi_3920 at 146.431 kb on - strand, within Dshi_3920 at 146.434 kb on + strand, within Dshi_3920 at 146.442 kb on - strand, within Dshi_3920 at 146.471 kb on - strand, within Dshi_3920 at 146.471 kb on - strand, within Dshi_3920 at 146.473 kb on + strand, within Dshi_3920 at 146.478 kb on + strand at 146.505 kb on - strand at 146.518 kb on + strand at 146.547 kb on - strand at 146.572 kb on + strand, within Dshi_3921 at 146.580 kb on - strand, within Dshi_3921 at 146.580 kb on - strand, within Dshi_3921 at 146.585 kb on - strand, within Dshi_3921 at 146.599 kb on - strand, within Dshi_3921 at 146.607 kb on - strand, within Dshi_3921 at 146.657 kb on - strand, within Dshi_3921 at 146.662 kb on - strand, within Dshi_3921 at 146.783 kb on - strand, within Dshi_3921 at 146.795 kb on + strand, within Dshi_3921 at 146.822 kb on + strand at 146.865 kb on - strand at 146.882 kb on - strand at 146.906 kb on - strand at 146.922 kb on + strand at 146.926 kb on - strand at 146.932 kb on - strand at 146.959 kb on + strand at 146.986 kb on + strand at 146.986 kb on + strand at 146.994 kb on - strand at 146.994 kb on - strand at 146.994 kb on - strand at 146.994 kb on - strand at 146.994 kb on - strand at 146.994 kb on - strand at 146.999 kb on - strand at 147.015 kb on + strand at 147.044 kb on + strand at 147.111 kb on - strand at 147.126 kb on + strand at 147.165 kb on + strand at 147.170 kb on - strand at 147.171 kb on - strand at 147.240 kb on + strand at 147.248 kb on - strand at 147.285 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 145,324 - Dshi_3918 0.66 +0.5 145,356 + Dshi_3918 0.73 -1.7 145,356 - Dshi_3918 0.73 +3.2 145,387 + Dshi_3918 0.80 +0.7 145,417 - Dshi_3918 0.86 +0.5 145,429 + Dshi_3918 0.89 -0.8 145,431 + Dshi_3918 0.89 -0.7 145,431 + Dshi_3918 0.89 -0.3 145,436 + -1.3 145,437 - +2.1 145,439 - +0.6 145,439 - -0.7 145,439 - +0.1 145,440 - +2.5 145,441 + +3.8 145,452 + +5.0 145,460 - -0.4 145,460 - -0.1 145,474 - +1.0 145,476 + -0.5 145,531 - +0.7 145,547 - -2.5 145,594 + +0.1 145,594 + +0.9 145,594 + -0.3 145,602 - +0.8 145,635 - +0.8 145,657 - -1.4 145,674 + -0.0 145,753 - +2.2 145,771 - +0.5 145,824 + -1.8 145,825 - +0.8 145,851 - Dshi_3919 0.14 +2.5 145,884 - Dshi_3919 0.23 -0.3 145,895 - Dshi_3919 0.26 -0.2 145,922 + Dshi_3919 0.34 -1.0 145,965 - Dshi_3919 0.45 +0.0 145,969 - Dshi_3919 0.46 +1.5 145,971 - Dshi_3919 0.47 +0.2 145,972 + Dshi_3919 0.47 +1.5 145,972 + Dshi_3919 0.47 +0.8 145,973 - Dshi_3919 0.47 +0.2 145,996 + Dshi_3919 0.54 -2.3 146,001 + Dshi_3919 0.55 +0.2 146,099 + Dshi_3919 0.82 -0.4 146,121 + Dshi_3919 0.88 +0.2 146,199 + +0.2 146,227 + +0.2 146,235 + +0.1 146,237 - -0.2 146,242 - -0.9 146,272 + -0.1 146,276 + +0.3 146,280 - +1.2 146,287 + -2.3 146,312 + +1.2 146,333 + Dshi_3920 0.17 -2.3 146,431 - Dshi_3920 0.67 +0.5 146,434 + Dshi_3920 0.68 +0.9 146,442 - Dshi_3920 0.72 +0.0 146,471 - Dshi_3920 0.87 -0.1 146,471 - Dshi_3920 0.87 +0.6 146,473 + Dshi_3920 0.88 -1.4 146,478 + +0.6 146,505 - +0.6 146,518 + +2.6 146,547 - -0.9 146,572 + Dshi_3921 0.16 +1.3 146,580 - Dshi_3921 0.18 +0.5 146,580 - Dshi_3921 0.18 -0.3 146,585 - Dshi_3921 0.20 +0.7 146,599 - Dshi_3921 0.24 -0.7 146,607 - Dshi_3921 0.27 -0.7 146,657 - Dshi_3921 0.43 -3.1 146,662 - Dshi_3921 0.44 -1.3 146,783 - Dshi_3921 0.83 -0.5 146,795 + Dshi_3921 0.86 -0.7 146,822 + +1.3 146,865 - +1.1 146,882 - -0.5 146,906 - -0.7 146,922 + -0.1 146,926 - +0.1 146,932 - +0.6 146,959 + +0.2 146,986 + -0.4 146,986 + +0.3 146,994 - -0.7 146,994 - -1.4 146,994 - -0.2 146,994 - +0.3 146,994 - +2.1 146,994 - +0.7 146,999 - -0.2 147,015 + -0.1 147,044 + +0.6 147,111 - +2.0 147,126 + +0.2 147,165 + +1.5 147,170 - -0.2 147,171 - -0.0 147,240 + -0.7 147,248 - -0.2 147,285 + +0.1
Or see this region's nucleotide sequence