Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3446 and Dshi_3447 are separated by 57 nucleotides Dshi_3447 and Dshi_3448 are separated by 4 nucleotides Dshi_3448 and Dshi_3449 overlap by 8 nucleotides Dshi_3449 and Dshi_3450 overlap by 4 nucleotides
Dshi_3446: Dshi_3446 - FxsA cytoplasmic membrane protein (RefSeq), at 3,629,595 to 3,630,086
_3446
Dshi_3447: Dshi_3447 - protein-export protein SecB (RefSeq), at 3,630,144 to 3,630,662
_3447
Dshi_3448: Dshi_3448 - DNA polymerase III, epsilon subunit (RefSeq), at 3,630,667 to 3,631,359
_3448
Dshi_3449: Dshi_3449 - dephospho-CoA kinase (RefSeq), at 3,631,352 to 3,631,948
_3449
Dshi_3450: Dshi_3450 - shikimate 5-dehydrogenase (RefSeq), at 3,631,945 to 3,632,781
_3450
Position (kb)
3630
3631
3632 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3629.673 kb on - strand, within Dshi_3446 at 3629.673 kb on - strand, within Dshi_3446 at 3629.677 kb on + strand, within Dshi_3446 at 3629.677 kb on + strand, within Dshi_3446 at 3629.685 kb on - strand, within Dshi_3446 at 3629.685 kb on - strand, within Dshi_3446 at 3629.711 kb on + strand, within Dshi_3446 at 3629.711 kb on + strand, within Dshi_3446 at 3629.711 kb on + strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.729 kb on - strand, within Dshi_3446 at 3629.729 kb on - strand, within Dshi_3446 at 3629.729 kb on - strand, within Dshi_3446 at 3629.748 kb on - strand, within Dshi_3446 at 3629.771 kb on - strand, within Dshi_3446 at 3629.799 kb on + strand, within Dshi_3446 at 3629.808 kb on - strand, within Dshi_3446 at 3629.833 kb on - strand, within Dshi_3446 at 3629.838 kb on + strand, within Dshi_3446 at 3629.838 kb on + strand, within Dshi_3446 at 3629.838 kb on + strand, within Dshi_3446 at 3629.846 kb on - strand, within Dshi_3446 at 3629.868 kb on + strand, within Dshi_3446 at 3629.920 kb on - strand, within Dshi_3446 at 3629.933 kb on + strand, within Dshi_3446 at 3629.975 kb on - strand, within Dshi_3446 at 3629.983 kb on + strand, within Dshi_3446 at 3629.991 kb on - strand, within Dshi_3446 at 3630.033 kb on + strand, within Dshi_3446 at 3630.038 kb on - strand at 3630.049 kb on + strand at 3630.088 kb on - strand at 3630.273 kb on + strand, within Dshi_3447 at 3630.273 kb on + strand, within Dshi_3447 at 3630.273 kb on + strand, within Dshi_3447 at 3630.274 kb on - strand, within Dshi_3447 at 3630.277 kb on - strand, within Dshi_3447 at 3630.281 kb on - strand, within Dshi_3447 at 3630.301 kb on + strand, within Dshi_3447 at 3630.325 kb on - strand, within Dshi_3447 at 3630.415 kb on + strand, within Dshi_3447 at 3630.470 kb on + strand, within Dshi_3447 at 3630.478 kb on - strand, within Dshi_3447 at 3630.496 kb on - strand, within Dshi_3447 at 3630.579 kb on - strand, within Dshi_3447 at 3630.628 kb on - strand at 3630.656 kb on + strand at 3630.817 kb on + strand, within Dshi_3448 at 3631.179 kb on + strand, within Dshi_3448 at 3631.185 kb on + strand, within Dshi_3448 at 3631.228 kb on + strand, within Dshi_3448 at 3631.299 kb on + strand at 3631.364 kb on + strand at 3631.367 kb on - strand at 3631.369 kb on + strand at 3631.369 kb on - strand at 3631.377 kb on - strand at 3631.379 kb on + strand at 3631.379 kb on + strand at 3631.387 kb on - strand at 3631.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 3,629,673 - Dshi_3446 0.16 +0.6 3,629,673 - Dshi_3446 0.16 +2.9 3,629,677 + Dshi_3446 0.17 +0.4 3,629,677 + Dshi_3446 0.17 +0.8 3,629,685 - Dshi_3446 0.18 -0.6 3,629,685 - Dshi_3446 0.18 -1.6 3,629,711 + Dshi_3446 0.24 +0.7 3,629,711 + Dshi_3446 0.24 -0.6 3,629,711 + Dshi_3446 0.24 -1.0 3,629,719 - Dshi_3446 0.25 -0.0 3,629,719 - Dshi_3446 0.25 +0.4 3,629,719 - Dshi_3446 0.25 -0.4 3,629,719 - Dshi_3446 0.25 -0.3 3,629,729 - Dshi_3446 0.27 +0.3 3,629,729 - Dshi_3446 0.27 +0.8 3,629,729 - Dshi_3446 0.27 -0.5 3,629,748 - Dshi_3446 0.31 -0.6 3,629,771 - Dshi_3446 0.36 -1.3 3,629,799 + Dshi_3446 0.41 -0.1 3,629,808 - Dshi_3446 0.43 -1.3 3,629,833 - Dshi_3446 0.48 +0.1 3,629,838 + Dshi_3446 0.49 +0.6 3,629,838 + Dshi_3446 0.49 +0.1 3,629,838 + Dshi_3446 0.49 +0.3 3,629,846 - Dshi_3446 0.51 -1.9 3,629,868 + Dshi_3446 0.55 -0.5 3,629,920 - Dshi_3446 0.66 -0.2 3,629,933 + Dshi_3446 0.69 -1.3 3,629,975 - Dshi_3446 0.77 -0.2 3,629,983 + Dshi_3446 0.79 +0.8 3,629,991 - Dshi_3446 0.80 +2.4 3,630,033 + Dshi_3446 0.89 -0.4 3,630,038 - -3.6 3,630,049 + +0.1 3,630,088 - +0.3 3,630,273 + Dshi_3447 0.25 -1.2 3,630,273 + Dshi_3447 0.25 -1.7 3,630,273 + Dshi_3447 0.25 -1.8 3,630,274 - Dshi_3447 0.25 -1.0 3,630,277 - Dshi_3447 0.26 +0.5 3,630,281 - Dshi_3447 0.26 -0.2 3,630,301 + Dshi_3447 0.30 +0.5 3,630,325 - Dshi_3447 0.35 -2.9 3,630,415 + Dshi_3447 0.52 +1.0 3,630,470 + Dshi_3447 0.63 +0.5 3,630,478 - Dshi_3447 0.64 -1.6 3,630,496 - Dshi_3447 0.68 -1.1 3,630,579 - Dshi_3447 0.84 -0.1 3,630,628 - +0.2 3,630,656 + +0.6 3,630,817 + Dshi_3448 0.22 +0.2 3,631,179 + Dshi_3448 0.74 -0.1 3,631,185 + Dshi_3448 0.75 -2.0 3,631,228 + Dshi_3448 0.81 -2.6 3,631,299 + -0.4 3,631,364 + +1.4 3,631,367 - -1.5 3,631,369 + +0.5 3,631,369 - +2.9 3,631,377 - +3.5 3,631,379 + +0.2 3,631,379 + -1.4 3,631,387 - +1.0 3,631,387 - -1.1
Or see this region's nucleotide sequence