Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3217 and Dshi_3218 overlap by 4 nucleotides Dshi_3218 and Dshi_3219 overlap by 4 nucleotides Dshi_3219 and Dshi_3220 are separated by 4 nucleotides Dshi_3220 and Dshi_3221 are separated by 31 nucleotides
Dshi_3217: Dshi_3217 - CRISPR-associated protein, Cse4 family (RefSeq), at 3,386,358 to 3,387,479
_3217
Dshi_3218: Dshi_3218 - CRISPR-associated protein Cas5 family (RefSeq), at 3,387,476 to 3,388,162
_3218
Dshi_3219: Dshi_3219 - CRISPR-associated protein CT1974 (RefSeq), at 3,388,159 to 3,388,950
_3219
Dshi_3220: Dshi_3220 - CRISPR-associated protein Cas1 (RefSeq), at 3,388,955 to 3,389,830
_3220
Dshi_3221: Dshi_3221 - CRISPR-associated protein Cas2 (RefSeq), at 3,389,862 to 3,390,155
_3221
Position (kb)
3388
3389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3387.175 kb on + strand, within Dshi_3217 at 3387.187 kb on + strand, within Dshi_3217 at 3387.195 kb on - strand, within Dshi_3217 at 3387.288 kb on - strand, within Dshi_3217 at 3387.415 kb on - strand at 3387.546 kb on + strand, within Dshi_3218 at 3387.546 kb on + strand, within Dshi_3218 at 3387.554 kb on - strand, within Dshi_3218 at 3387.576 kb on + strand, within Dshi_3218 at 3387.707 kb on - strand, within Dshi_3218 at 3387.748 kb on + strand, within Dshi_3218 at 3387.756 kb on - strand, within Dshi_3218 at 3387.756 kb on - strand, within Dshi_3218 at 3387.847 kb on - strand, within Dshi_3218 at 3387.849 kb on - strand, within Dshi_3218 at 3387.895 kb on + strand, within Dshi_3218 at 3387.903 kb on - strand, within Dshi_3218 at 3387.918 kb on - strand, within Dshi_3218 at 3387.991 kb on - strand, within Dshi_3218 at 3388.031 kb on + strand, within Dshi_3218 at 3388.078 kb on - strand, within Dshi_3218 at 3388.105 kb on + strand at 3388.202 kb on - strand at 3388.221 kb on - strand at 3388.221 kb on - strand at 3388.260 kb on + strand, within Dshi_3219 at 3388.286 kb on - strand, within Dshi_3219 at 3388.365 kb on - strand, within Dshi_3219 at 3388.445 kb on + strand, within Dshi_3219 at 3388.453 kb on - strand, within Dshi_3219 at 3388.545 kb on + strand, within Dshi_3219 at 3388.551 kb on + strand, within Dshi_3219 at 3388.635 kb on + strand, within Dshi_3219 at 3388.662 kb on - strand, within Dshi_3219 at 3388.662 kb on - strand, within Dshi_3219 at 3388.909 kb on + strand at 3389.012 kb on + strand at 3389.012 kb on + strand at 3389.097 kb on + strand, within Dshi_3220 at 3389.181 kb on - strand, within Dshi_3220 at 3389.189 kb on - strand, within Dshi_3220 at 3389.242 kb on + strand, within Dshi_3220 at 3389.305 kb on - strand, within Dshi_3220 at 3389.310 kb on - strand, within Dshi_3220 at 3389.322 kb on + strand, within Dshi_3220 at 3389.341 kb on + strand, within Dshi_3220 at 3389.344 kb on + strand, within Dshi_3220 at 3389.380 kb on - strand, within Dshi_3220 at 3389.393 kb on - strand, within Dshi_3220 at 3389.419 kb on + strand, within Dshi_3220 at 3389.502 kb on + strand, within Dshi_3220 at 3389.519 kb on - strand, within Dshi_3220 at 3389.600 kb on + strand, within Dshi_3220 at 3389.674 kb on + strand, within Dshi_3220 at 3389.807 kb on + strand at 3389.824 kb on + strand at 3389.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 3,387,175 + Dshi_3217 0.73 +0.6 3,387,187 + Dshi_3217 0.74 -0.4 3,387,195 - Dshi_3217 0.75 -1.5 3,387,288 - Dshi_3217 0.83 -0.6 3,387,415 - -1.1 3,387,546 + Dshi_3218 0.10 +2.9 3,387,546 + Dshi_3218 0.10 -0.3 3,387,554 - Dshi_3218 0.11 -1.1 3,387,576 + Dshi_3218 0.15 +1.6 3,387,707 - Dshi_3218 0.34 +2.6 3,387,748 + Dshi_3218 0.40 +0.9 3,387,756 - Dshi_3218 0.41 -1.0 3,387,756 - Dshi_3218 0.41 +2.0 3,387,847 - Dshi_3218 0.54 -0.0 3,387,849 - Dshi_3218 0.54 -1.4 3,387,895 + Dshi_3218 0.61 -0.4 3,387,903 - Dshi_3218 0.62 -0.0 3,387,918 - Dshi_3218 0.64 -1.4 3,387,991 - Dshi_3218 0.75 -1.5 3,388,031 + Dshi_3218 0.81 +2.9 3,388,078 - Dshi_3218 0.88 +0.6 3,388,105 + -0.4 3,388,202 - +1.9 3,388,221 - -0.0 3,388,221 - +1.4 3,388,260 + Dshi_3219 0.13 +0.0 3,388,286 - Dshi_3219 0.16 -1.0 3,388,365 - Dshi_3219 0.26 +0.6 3,388,445 + Dshi_3219 0.36 -1.7 3,388,453 - Dshi_3219 0.37 +0.6 3,388,545 + Dshi_3219 0.49 +0.0 3,388,551 + Dshi_3219 0.49 +0.0 3,388,635 + Dshi_3219 0.60 -3.0 3,388,662 - Dshi_3219 0.64 -3.1 3,388,662 - Dshi_3219 0.64 +1.0 3,388,909 + -0.7 3,389,012 + -0.4 3,389,012 + -2.2 3,389,097 + Dshi_3220 0.16 +1.1 3,389,181 - Dshi_3220 0.26 -0.4 3,389,189 - Dshi_3220 0.27 +1.6 3,389,242 + Dshi_3220 0.33 -2.4 3,389,305 - Dshi_3220 0.40 -2.2 3,389,310 - Dshi_3220 0.41 +3.1 3,389,322 + Dshi_3220 0.42 +1.1 3,389,341 + Dshi_3220 0.44 -2.0 3,389,344 + Dshi_3220 0.44 +1.6 3,389,380 - Dshi_3220 0.49 -1.0 3,389,393 - Dshi_3220 0.50 +2.1 3,389,419 + Dshi_3220 0.53 +0.6 3,389,502 + Dshi_3220 0.62 +1.1 3,389,519 - Dshi_3220 0.64 +0.9 3,389,600 + Dshi_3220 0.74 +0.4 3,389,674 + Dshi_3220 0.82 -3.2 3,389,807 + +1.0 3,389,824 + -1.4 3,389,829 - +0.6
Or see this region's nucleotide sequence