Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2249 and Dshi_2250 overlap by 4 nucleotides Dshi_2250 and Dshi_2251 are separated by 2 nucleotides Dshi_2251 and Dshi_2252 are separated by 138 nucleotides Dshi_2252 and Dshi_2253 are separated by 186 nucleotides
Dshi_2249: Dshi_2249 - NusB antitermination factor (RefSeq), at 2,370,535 to 2,371,014
_2249
Dshi_2250: Dshi_2250 - 6,7-dimethyl-8-ribityllumazine synthase (RefSeq), at 2,371,011 to 2,371,562
_2250
Dshi_2251: Dshi_2251 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (RefSeq), at 2,371,565 to 2,372,680
_2251
Dshi_2252: Dshi_2252 - riboflavin synthase, alpha subunit (RefSeq), at 2,372,819 to 2,373,421
_2252
Dshi_2253: Dshi_2253 - Capsule polysaccharide biosynthesis protein (RefSeq), at 2,373,608 to 2,374,894
_2253
Position (kb)
2371
2372
2373 Strain fitness (log2 ratio)
-2
-1
0
1 at 2372.769 kb on - strand at 2373.422 kb on + strand at 2373.429 kb on - strand at 2373.429 kb on - strand at 2373.447 kb on - strand at 2373.474 kb on + strand at 2373.485 kb on + strand at 2373.493 kb on - strand at 2373.545 kb on - strand at 2373.604 kb on + strand at 2373.606 kb on - strand at 2373.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 2,372,769 - -1.1 2,373,422 + -0.2 2,373,429 - +0.0 2,373,429 - +0.8 2,373,447 - -1.0 2,373,474 + -1.8 2,373,485 + +0.3 2,373,493 - -1.0 2,373,545 - -0.7 2,373,604 + -0.8 2,373,606 - +1.1 2,373,612 - -0.3
Or see this region's nucleotide sequence