Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2033 and Dshi_2034 are separated by 9 nucleotides Dshi_2034 and Dshi_2035 overlap by 8 nucleotides Dshi_2035 and Dshi_2036 overlap by 4 nucleotides
Dshi_2033: Dshi_2033 - TRAP dicarboxylate transporter, DctM subunit (RefSeq), at 2,152,380 to 2,153,708
_2033
Dshi_2034: Dshi_2034 - Histidinol dehydrogenase (RefSeq), at 2,153,718 to 2,155,025
_2034
Dshi_2035: Dshi_2035 - short-chain dehydrogenase/reductase SDR (RefSeq), at 2,155,018 to 2,155,782
_2035
Dshi_2036: Dshi_2036 - Alcohol dehydrogenase GroES domain protein (RefSeq), at 2,155,779 to 2,156,762
_2036
Position (kb)
2153
2154
2155
2156 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2152.767 kb on + strand, within Dshi_2033 at 2152.861 kb on + strand, within Dshi_2033 at 2152.923 kb on + strand, within Dshi_2033 at 2152.931 kb on - strand, within Dshi_2033 at 2153.054 kb on + strand, within Dshi_2033 at 2153.071 kb on + strand, within Dshi_2033 at 2153.072 kb on - strand, within Dshi_2033 at 2153.208 kb on - strand, within Dshi_2033 at 2153.208 kb on - strand, within Dshi_2033 at 2153.246 kb on + strand, within Dshi_2033 at 2153.254 kb on - strand, within Dshi_2033 at 2153.523 kb on - strand, within Dshi_2033 at 2153.529 kb on + strand, within Dshi_2033 at 2153.551 kb on + strand, within Dshi_2033 at 2153.551 kb on + strand, within Dshi_2033 at 2153.559 kb on - strand, within Dshi_2033 at 2153.559 kb on - strand, within Dshi_2033 at 2153.559 kb on - strand, within Dshi_2033 at 2153.559 kb on - strand, within Dshi_2033 at 2153.581 kb on - strand at 2153.738 kb on - strand at 2153.753 kb on - strand at 2153.754 kb on + strand at 2153.769 kb on + strand at 2153.817 kb on + strand at 2153.827 kb on + strand at 2153.832 kb on + strand at 2153.892 kb on - strand, within Dshi_2034 at 2153.901 kb on - strand, within Dshi_2034 at 2153.979 kb on + strand, within Dshi_2034 at 2154.021 kb on - strand, within Dshi_2034 at 2154.027 kb on - strand, within Dshi_2034 at 2154.188 kb on - strand, within Dshi_2034 at 2154.218 kb on + strand, within Dshi_2034 at 2154.228 kb on + strand, within Dshi_2034 at 2154.453 kb on - strand, within Dshi_2034 at 2154.468 kb on + strand, within Dshi_2034 at 2154.541 kb on + strand, within Dshi_2034 at 2154.547 kb on - strand, within Dshi_2034 at 2154.623 kb on - strand, within Dshi_2034 at 2154.628 kb on - strand, within Dshi_2034 at 2154.675 kb on - strand, within Dshi_2034 at 2154.755 kb on + strand, within Dshi_2034 at 2154.787 kb on - strand, within Dshi_2034 at 2154.862 kb on - strand, within Dshi_2034 at 2154.862 kb on - strand, within Dshi_2034 at 2154.893 kb on + strand, within Dshi_2034 at 2154.893 kb on + strand, within Dshi_2034 at 2154.901 kb on - strand at 2154.948 kb on - strand at 2154.949 kb on + strand at 2154.953 kb on + strand at 2155.008 kb on - strand at 2155.048 kb on + strand at 2155.058 kb on - strand at 2155.294 kb on - strand, within Dshi_2035 at 2155.330 kb on - strand, within Dshi_2035 at 2155.482 kb on - strand, within Dshi_2035 at 2155.514 kb on - strand, within Dshi_2035 at 2155.550 kb on - strand, within Dshi_2035 at 2155.613 kb on - strand, within Dshi_2035 at 2155.636 kb on + strand, within Dshi_2035 at 2155.798 kb on - strand at 2155.865 kb on - strand at 2155.938 kb on - strand, within Dshi_2036
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 2,152,767 + Dshi_2033 0.29 +0.9 2,152,861 + Dshi_2033 0.36 -2.2 2,152,923 + Dshi_2033 0.41 -0.2 2,152,931 - Dshi_2033 0.41 -1.6 2,153,054 + Dshi_2033 0.51 -2.4 2,153,071 + Dshi_2033 0.52 -1.2 2,153,072 - Dshi_2033 0.52 +1.4 2,153,208 - Dshi_2033 0.62 -1.2 2,153,208 - Dshi_2033 0.62 -0.5 2,153,246 + Dshi_2033 0.65 -2.0 2,153,254 - Dshi_2033 0.66 -2.6 2,153,523 - Dshi_2033 0.86 +0.1 2,153,529 + Dshi_2033 0.86 +0.4 2,153,551 + Dshi_2033 0.88 -0.4 2,153,551 + Dshi_2033 0.88 -1.6 2,153,559 - Dshi_2033 0.89 +2.4 2,153,559 - Dshi_2033 0.89 -0.9 2,153,559 - Dshi_2033 0.89 -1.6 2,153,559 - Dshi_2033 0.89 -0.1 2,153,581 - -2.5 2,153,738 - -3.0 2,153,753 - +3.2 2,153,754 + +0.5 2,153,769 + -2.8 2,153,817 + -1.3 2,153,827 + +0.6 2,153,832 + -2.3 2,153,892 - Dshi_2034 0.13 -0.8 2,153,901 - Dshi_2034 0.14 -0.6 2,153,979 + Dshi_2034 0.20 -1.2 2,154,021 - Dshi_2034 0.23 -4.0 2,154,027 - Dshi_2034 0.24 +0.1 2,154,188 - Dshi_2034 0.36 +0.0 2,154,218 + Dshi_2034 0.38 +2.4 2,154,228 + Dshi_2034 0.39 +1.4 2,154,453 - Dshi_2034 0.56 +0.4 2,154,468 + Dshi_2034 0.57 +0.4 2,154,541 + Dshi_2034 0.63 +2.4 2,154,547 - Dshi_2034 0.63 +4.2 2,154,623 - Dshi_2034 0.69 -0.6 2,154,628 - Dshi_2034 0.70 -0.6 2,154,675 - Dshi_2034 0.73 -1.4 2,154,755 + Dshi_2034 0.79 +0.4 2,154,787 - Dshi_2034 0.82 -0.7 2,154,862 - Dshi_2034 0.87 -0.2 2,154,862 - Dshi_2034 0.87 -0.8 2,154,893 + Dshi_2034 0.90 -0.5 2,154,893 + Dshi_2034 0.90 +0.4 2,154,901 - -0.4 2,154,948 - -0.9 2,154,949 + +0.4 2,154,953 + +0.4 2,155,008 - -0.0 2,155,048 + +0.4 2,155,058 - -0.4 2,155,294 - Dshi_2035 0.36 -2.4 2,155,330 - Dshi_2035 0.41 -0.6 2,155,482 - Dshi_2035 0.61 +0.6 2,155,514 - Dshi_2035 0.65 -3.1 2,155,550 - Dshi_2035 0.70 -0.3 2,155,613 - Dshi_2035 0.78 -2.8 2,155,636 + Dshi_2035 0.81 -0.5 2,155,798 - +1.2 2,155,865 - -0.0 2,155,938 - Dshi_2036 0.16 +1.0
Or see this region's nucleotide sequence