Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1684 and Dshi_1685 are separated by 242 nucleotides Dshi_1685 and Dshi_1686 are separated by 10 nucleotides Dshi_1686 and Dshi_1687 are separated by 127 nucleotides
Dshi_1684: Dshi_1684 - glucose-6-phosphate 1-dehydrogenase (RefSeq), at 1,753,116 to 1,754,570
_1684
Dshi_1685: Dshi_1685 - Radical SAM domain protein (RefSeq), at 1,754,813 to 1,755,757
_1685
Dshi_1686: Dshi_1686 - transglutaminase domain protein (RefSeq), at 1,755,768 to 1,756,544
_1686
Dshi_1687: Dshi_1687 - HNH endonuclease (RefSeq), at 1,756,672 to 1,757,268
_1687
Position (kb)
1754
1755
1756 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1754.586 kb on - strand at 1754.587 kb on + strand at 1754.629 kb on + strand at 1754.723 kb on + strand at 1754.760 kb on - strand at 1754.878 kb on - strand at 1754.930 kb on - strand, within Dshi_1685 at 1754.986 kb on - strand, within Dshi_1685 at 1754.992 kb on + strand, within Dshi_1685 at 1755.001 kb on - strand, within Dshi_1685 at 1755.001 kb on - strand, within Dshi_1685 at 1755.054 kb on - strand, within Dshi_1685 at 1755.115 kb on + strand, within Dshi_1685 at 1755.138 kb on + strand, within Dshi_1685 at 1755.255 kb on - strand, within Dshi_1685 at 1755.421 kb on + strand, within Dshi_1685 at 1755.461 kb on - strand, within Dshi_1685 at 1755.498 kb on + strand, within Dshi_1685 at 1755.728 kb on + strand at 1755.856 kb on + strand, within Dshi_1686 at 1755.925 kb on + strand, within Dshi_1686 at 1756.017 kb on - strand, within Dshi_1686 at 1756.161 kb on - strand, within Dshi_1686 at 1756.182 kb on - strand, within Dshi_1686 at 1756.323 kb on - strand, within Dshi_1686 at 1756.323 kb on - strand, within Dshi_1686 at 1756.334 kb on + strand, within Dshi_1686 at 1756.352 kb on - strand, within Dshi_1686 at 1756.492 kb on + strand at 1756.539 kb on - strand at 1756.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 1,754,586 - -2.3 1,754,587 + -1.1 1,754,629 + -0.6 1,754,723 + -1.0 1,754,760 - +0.2 1,754,878 - -2.6 1,754,930 - Dshi_1685 0.12 +1.2 1,754,986 - Dshi_1685 0.18 -0.8 1,754,992 + Dshi_1685 0.19 -0.1 1,755,001 - Dshi_1685 0.20 +1.6 1,755,001 - Dshi_1685 0.20 +2.0 1,755,054 - Dshi_1685 0.26 -0.3 1,755,115 + Dshi_1685 0.32 +1.1 1,755,138 + Dshi_1685 0.34 -1.9 1,755,255 - Dshi_1685 0.47 +1.5 1,755,421 + Dshi_1685 0.64 -0.7 1,755,461 - Dshi_1685 0.69 +0.4 1,755,498 + Dshi_1685 0.72 -1.2 1,755,728 + +1.2 1,755,856 + Dshi_1686 0.11 -1.5 1,755,925 + Dshi_1686 0.20 -0.8 1,756,017 - Dshi_1686 0.32 -0.1 1,756,161 - Dshi_1686 0.51 +1.7 1,756,182 - Dshi_1686 0.53 +3.0 1,756,323 - Dshi_1686 0.71 -1.6 1,756,323 - Dshi_1686 0.71 -0.8 1,756,334 + Dshi_1686 0.73 -2.6 1,756,352 - Dshi_1686 0.75 +0.5 1,756,492 + -1.1 1,756,539 - +0.5 1,756,723 - -1.6
Or see this region's nucleotide sequence