Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0984 and Dshi_R0023 are separated by 292 nucleotides Dshi_R0023 and Dshi_0985 are separated by 423 nucleotides Dshi_0985 and Dshi_0986 are separated by 177 nucleotides
Dshi_0984: Dshi_0984 - ribonuclease (RefSeq), at 1,003,740 to 1,004,783
_0984
Dshi_R0023: Dshi_R0023 - tRNA-Phe (RefSeq), at 1,005,076 to 1,005,150
_R0023
Dshi_0985: Dshi_0985 - hypothetical protein (RefSeq), at 1,005,574 to 1,005,831
_0985
Dshi_0986: Dshi_0986 - helix-turn-helix domain protein (RefSeq), at 1,006,009 to 1,006,383
_0986
Position (kb)
1005
1006 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1004.191 kb on + strand, within Dshi_0984 at 1004.260 kb on - strand, within Dshi_0984 at 1004.260 kb on - strand, within Dshi_0984 at 1004.260 kb on - strand, within Dshi_0984 at 1004.297 kb on + strand, within Dshi_0984 at 1004.431 kb on + strand, within Dshi_0984 at 1004.444 kb on + strand, within Dshi_0984 at 1004.444 kb on + strand, within Dshi_0984 at 1004.484 kb on - strand, within Dshi_0984 at 1004.598 kb on + strand, within Dshi_0984 at 1004.598 kb on - strand, within Dshi_0984 at 1004.693 kb on + strand at 1004.715 kb on + strand at 1004.716 kb on + strand at 1004.725 kb on + strand at 1004.785 kb on - strand at 1004.787 kb on + strand at 1004.818 kb on - strand at 1004.863 kb on - strand at 1004.928 kb on - strand at 1004.980 kb on + strand at 1005.318 kb on - strand at 1005.322 kb on + strand at 1005.358 kb on - strand at 1005.574 kb on - strand at 1005.593 kb on - strand at 1005.596 kb on + strand at 1005.695 kb on + strand, within Dshi_0985 at 1005.731 kb on - strand, within Dshi_0985 at 1005.731 kb on - strand, within Dshi_0985 at 1005.743 kb on + strand, within Dshi_0985 at 1005.748 kb on + strand, within Dshi_0985 at 1005.748 kb on + strand, within Dshi_0985 at 1005.808 kb on - strand at 1005.819 kb on + strand at 1005.860 kb on + strand at 1005.916 kb on + strand at 1005.939 kb on + strand at 1005.967 kb on + strand at 1006.002 kb on - strand at 1006.017 kb on + strand at 1006.034 kb on + strand at 1006.040 kb on + strand at 1006.040 kb on + strand at 1006.042 kb on - strand at 1006.144 kb on + strand, within Dshi_0986
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 1,004,191 + Dshi_0984 0.43 +0.1 1,004,260 - Dshi_0984 0.50 +0.1 1,004,260 - Dshi_0984 0.50 +0.8 1,004,260 - Dshi_0984 0.50 -0.0 1,004,297 + Dshi_0984 0.53 -0.8 1,004,431 + Dshi_0984 0.66 +0.8 1,004,444 + Dshi_0984 0.67 +0.8 1,004,444 + Dshi_0984 0.67 +0.4 1,004,484 - Dshi_0984 0.71 +2.3 1,004,598 + Dshi_0984 0.82 -0.8 1,004,598 - Dshi_0984 0.82 +0.6 1,004,693 + -1.0 1,004,715 + -0.9 1,004,716 + +0.6 1,004,725 + +1.8 1,004,785 - -0.5 1,004,787 + +2.0 1,004,818 - +0.8 1,004,863 - +2.2 1,004,928 - +0.8 1,004,980 + +0.0 1,005,318 - +1.0 1,005,322 + -0.6 1,005,358 - +0.8 1,005,574 - +1.3 1,005,593 - -0.0 1,005,596 + -0.1 1,005,695 + Dshi_0985 0.47 +1.3 1,005,731 - Dshi_0985 0.61 +0.2 1,005,731 - Dshi_0985 0.61 +0.4 1,005,743 + Dshi_0985 0.66 -0.3 1,005,748 + Dshi_0985 0.67 -2.6 1,005,748 + Dshi_0985 0.67 +0.6 1,005,808 - +0.3 1,005,819 + -0.5 1,005,860 + -0.9 1,005,916 + -1.7 1,005,939 + -0.9 1,005,967 + -0.3 1,006,002 - +0.4 1,006,017 + -3.9 1,006,034 + +3.2 1,006,040 + -0.4 1,006,040 + -3.1 1,006,042 - -0.2 1,006,144 + Dshi_0986 0.36 +1.9
Or see this region's nucleotide sequence