Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0911 and Dshi_0912 overlap by 4 nucleotides Dshi_0912 and Dshi_0913 are separated by 3 nucleotides Dshi_0913 and Dshi_0914 overlap by 17 nucleotides Dshi_0914 and Dshi_0915 overlap by 4 nucleotides
Dshi_0911: Dshi_0911 - Thiamine-phosphate diphosphorylase (RefSeq), at 934,241 to 934,840
_0911
Dshi_0912: Dshi_0912 - thiazole biosynthesis family protein (RefSeq), at 934,837 to 935,631
_0912
Dshi_0913: Dshi_0913 - thiamine biosynthesis protein ThiS (RefSeq), at 935,635 to 935,829
_0913
Dshi_0914: Dshi_0914 - glycine oxidase ThiO (RefSeq), at 935,813 to 936,790
_0914
Dshi_0915: Dshi_0915 - phosphomethylpyrimidine kinase (RefSeq), at 936,787 to 937,584
_0915
Position (kb) 935
936
937 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 935.087 kb on - strand, within Dshi_0912 at 935.127 kb on + strand, within Dshi_0912 at 935.169 kb on - strand, within Dshi_0912 at 935.274 kb on + strand, within Dshi_0912 at 935.376 kb on - strand, within Dshi_0912 at 935.457 kb on - strand, within Dshi_0912 at 935.621 kb on + strand at 935.621 kb on + strand at 935.694 kb on - strand, within Dshi_0913 at 935.884 kb on + strand at 936.063 kb on - strand, within Dshi_0914 at 936.195 kb on - strand, within Dshi_0914 at 936.394 kb on - strand, within Dshi_0914 at 936.573 kb on + strand, within Dshi_0914 at 936.607 kb on - strand, within Dshi_0914 at 936.668 kb on + strand, within Dshi_0914 at 936.885 kb on - strand, within Dshi_0915 at 936.914 kb on + strand, within Dshi_0915 at 937.069 kb on + strand, within Dshi_0915 at 937.166 kb on + strand, within Dshi_0915 at 937.176 kb on + strand, within Dshi_0915 at 937.209 kb on - strand, within Dshi_0915 at 937.335 kb on + strand, within Dshi_0915 at 937.348 kb on - strand, within Dshi_0915 at 937.477 kb on + strand, within Dshi_0915 at 937.621 kb on - strand at 937.785 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 935,087 - Dshi_0912 0.31 +0.8 935,127 + Dshi_0912 0.36 +0.8 935,169 - Dshi_0912 0.42 +1.4 935,274 + Dshi_0912 0.55 +0.1 935,376 - Dshi_0912 0.68 -0.5 935,457 - Dshi_0912 0.78 -1.6 935,621 + -1.3 935,621 + -1.9 935,694 - Dshi_0913 0.30 +2.0 935,884 + +0.4 936,063 - Dshi_0914 0.26 -0.6 936,195 - Dshi_0914 0.39 -0.5 936,394 - Dshi_0914 0.59 +1.4 936,573 + Dshi_0914 0.78 -2.9 936,607 - Dshi_0914 0.81 -1.6 936,668 + Dshi_0914 0.87 -0.6 936,885 - Dshi_0915 0.12 -0.2 936,914 + Dshi_0915 0.16 +1.4 937,069 + Dshi_0915 0.35 +0.4 937,166 + Dshi_0915 0.47 -0.3 937,176 + Dshi_0915 0.49 -0.8 937,209 - Dshi_0915 0.53 +0.4 937,335 + Dshi_0915 0.69 +3.0 937,348 - Dshi_0915 0.70 +3.7 937,477 + Dshi_0915 0.86 +2.7 937,621 - -1.2 937,785 - +1.4
Or see this region's nucleotide sequence