Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0524 and Dshi_0525 are separated by 83 nucleotides Dshi_0525 and Dshi_0526 are separated by 27 nucleotides Dshi_0526 and Dshi_0527 overlap by 4 nucleotides
Dshi_0524: Dshi_0524 - aldo/keto reductase (RefSeq), at 508,895 to 509,974
_0524
Dshi_0525: Dshi_0525 - hypothetical protein (RefSeq), at 510,058 to 510,609
_0525
Dshi_0526: Dshi_0526 - Dak phosphatase (RefSeq), at 510,637 to 511,236
_0526
Dshi_0527: Dshi_0527 - Glycerone kinase (RefSeq), at 511,233 to 512,234
_0527
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 509.691 kb on + strand, within Dshi_0524 at 509.696 kb on + strand, within Dshi_0524 at 509.860 kb on - strand, within Dshi_0524 at 509.876 kb on + strand at 509.897 kb on + strand at 509.912 kb on + strand at 509.963 kb on + strand at 509.976 kb on - strand at 509.976 kb on - strand at 509.977 kb on + strand at 510.029 kb on - strand at 510.120 kb on - strand, within Dshi_0525 at 510.168 kb on + strand, within Dshi_0525 at 510.186 kb on + strand, within Dshi_0525 at 510.192 kb on + strand, within Dshi_0525 at 510.192 kb on + strand, within Dshi_0525 at 510.192 kb on + strand, within Dshi_0525 at 510.202 kb on + strand, within Dshi_0525 at 510.210 kb on - strand, within Dshi_0525 at 510.219 kb on - strand, within Dshi_0525 at 510.280 kb on - strand, within Dshi_0525 at 510.280 kb on - strand, within Dshi_0525 at 510.339 kb on + strand, within Dshi_0525 at 510.571 kb on + strand at 510.579 kb on - strand at 510.611 kb on - strand at 510.628 kb on + strand at 510.656 kb on - strand at 510.689 kb on - strand at 510.772 kb on + strand, within Dshi_0526 at 510.780 kb on - strand, within Dshi_0526 at 510.786 kb on + strand, within Dshi_0526 at 510.787 kb on - strand, within Dshi_0526 at 510.796 kb on + strand, within Dshi_0526 at 510.796 kb on + strand, within Dshi_0526 at 510.799 kb on - strand, within Dshi_0526 at 510.844 kb on + strand, within Dshi_0526 at 510.867 kb on + strand, within Dshi_0526 at 510.885 kb on - strand, within Dshi_0526 at 510.905 kb on + strand, within Dshi_0526 at 510.905 kb on + strand, within Dshi_0526 at 510.920 kb on + strand, within Dshi_0526 at 510.981 kb on - strand, within Dshi_0526 at 511.029 kb on + strand, within Dshi_0526 at 511.034 kb on + strand, within Dshi_0526 at 511.140 kb on + strand, within Dshi_0526 at 511.154 kb on + strand, within Dshi_0526 at 511.162 kb on - strand, within Dshi_0526 at 511.165 kb on + strand, within Dshi_0526 at 511.246 kb on - strand at 511.265 kb on - strand at 511.307 kb on + strand at 511.307 kb on + strand at 511.315 kb on - strand at 511.317 kb on - strand at 511.373 kb on - strand, within Dshi_0527 at 511.397 kb on + strand, within Dshi_0527 at 511.397 kb on + strand, within Dshi_0527 at 511.405 kb on - strand, within Dshi_0527 at 511.407 kb on + strand, within Dshi_0527 at 511.407 kb on + strand, within Dshi_0527 at 511.417 kb on + strand, within Dshi_0527 at 511.424 kb on - strand, within Dshi_0527 at 511.436 kb on + strand, within Dshi_0527 at 511.436 kb on + strand, within Dshi_0527 at 511.540 kb on + strand, within Dshi_0527 at 511.540 kb on - strand, within Dshi_0527 at 511.542 kb on + strand, within Dshi_0527 at 511.566 kb on + strand, within Dshi_0527 at 511.598 kb on - strand, within Dshi_0527 at 511.631 kb on - strand, within Dshi_0527 at 511.645 kb on + strand, within Dshi_0527 at 511.645 kb on + strand, within Dshi_0527 at 511.693 kb on + strand, within Dshi_0527 at 511.719 kb on - strand, within Dshi_0527 at 511.761 kb on + strand, within Dshi_0527 at 511.841 kb on + strand, within Dshi_0527 at 511.875 kb on + strand, within Dshi_0527 at 511.934 kb on + strand, within Dshi_0527 at 512.016 kb on + strand, within Dshi_0527 at 512.121 kb on + strand, within Dshi_0527 at 512.129 kb on - strand, within Dshi_0527 at 512.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 509,691 + Dshi_0524 0.74 -1.0 509,696 + Dshi_0524 0.74 +0.4 509,860 - Dshi_0524 0.89 +0.4 509,876 + +2.0 509,897 + +1.1 509,912 + -0.7 509,963 + -0.8 509,976 - +3.2 509,976 - -1.1 509,977 + -0.3 510,029 - -0.5 510,120 - Dshi_0525 0.11 +0.3 510,168 + Dshi_0525 0.20 -1.0 510,186 + Dshi_0525 0.23 -0.8 510,192 + Dshi_0525 0.24 +0.5 510,192 + Dshi_0525 0.24 -0.8 510,192 + Dshi_0525 0.24 -0.2 510,202 + Dshi_0525 0.26 +3.8 510,210 - Dshi_0525 0.28 +0.3 510,219 - Dshi_0525 0.29 -0.6 510,280 - Dshi_0525 0.40 +0.1 510,280 - Dshi_0525 0.40 +2.2 510,339 + Dshi_0525 0.51 -0.3 510,571 + -0.2 510,579 - +3.6 510,611 - -1.7 510,628 + +0.2 510,656 - +2.6 510,689 - +3.3 510,772 + Dshi_0526 0.23 -0.8 510,780 - Dshi_0526 0.24 +0.1 510,786 + Dshi_0526 0.25 +0.8 510,787 - Dshi_0526 0.25 +0.4 510,796 + Dshi_0526 0.27 -0.2 510,796 + Dshi_0526 0.27 -0.2 510,799 - Dshi_0526 0.27 -2.8 510,844 + Dshi_0526 0.34 +2.6 510,867 + Dshi_0526 0.38 -0.5 510,885 - Dshi_0526 0.41 -0.1 510,905 + Dshi_0526 0.45 -0.1 510,905 + Dshi_0526 0.45 +0.0 510,920 + Dshi_0526 0.47 -0.2 510,981 - Dshi_0526 0.57 -0.4 511,029 + Dshi_0526 0.65 -1.0 511,034 + Dshi_0526 0.66 +0.3 511,140 + Dshi_0526 0.84 +0.7 511,154 + Dshi_0526 0.86 -1.5 511,162 - Dshi_0526 0.88 +0.0 511,165 + Dshi_0526 0.88 +0.1 511,246 - -0.1 511,265 - +0.2 511,307 + -0.0 511,307 + +0.1 511,315 - -0.6 511,317 - +0.4 511,373 - Dshi_0527 0.14 -1.0 511,397 + Dshi_0527 0.16 +0.4 511,397 + Dshi_0527 0.16 -0.6 511,405 - Dshi_0527 0.17 +0.1 511,407 + Dshi_0527 0.17 +0.4 511,407 + Dshi_0527 0.17 -0.5 511,417 + Dshi_0527 0.18 -0.1 511,424 - Dshi_0527 0.19 -1.8 511,436 + Dshi_0527 0.20 -2.6 511,436 + Dshi_0527 0.20 -2.0 511,540 + Dshi_0527 0.31 -0.4 511,540 - Dshi_0527 0.31 +2.1 511,542 + Dshi_0527 0.31 +1.2 511,566 + Dshi_0527 0.33 +0.5 511,598 - Dshi_0527 0.36 +0.7 511,631 - Dshi_0527 0.40 -1.1 511,645 + Dshi_0527 0.41 +0.8 511,645 + Dshi_0527 0.41 +0.9 511,693 + Dshi_0527 0.46 -0.3 511,719 - Dshi_0527 0.49 -0.5 511,761 + Dshi_0527 0.53 -0.4 511,841 + Dshi_0527 0.61 -0.9 511,875 + Dshi_0527 0.64 +0.5 511,934 + Dshi_0527 0.70 +0.0 512,016 + Dshi_0527 0.78 -0.2 512,121 + Dshi_0527 0.89 -0.4 512,129 - Dshi_0527 0.89 +0.0 512,190 + +1.0
Or see this region's nucleotide sequence