Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0339 and Dshi_0340 are separated by 117 nucleotides Dshi_0340 and Dshi_0341 are separated by 11 nucleotides Dshi_0341 and Dshi_0342 are separated by 260 nucleotides
Dshi_0339: Dshi_0339 - protein of unknown function DUF849 (RefSeq), at 327,660 to 328,538
_0339
Dshi_0340: Dshi_0340 - ribosomal protein L31 (RefSeq), at 328,656 to 328,877
_0340
Dshi_0341: Dshi_0341 - ribosomal protein L19 (RefSeq), at 328,889 to 329,263
_0341
Dshi_0342: Dshi_0342 - tRNA (guanine-N1)-methyltransferase (RefSeq), at 329,524 to 330,321
_0342
Position (kb) 328
329 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 327.759 kb on + strand, within Dshi_0339 at 327.786 kb on + strand, within Dshi_0339 at 327.786 kb on + strand, within Dshi_0339 at 327.794 kb on - strand, within Dshi_0339 at 327.794 kb on - strand, within Dshi_0339 at 327.834 kb on - strand, within Dshi_0339 at 327.920 kb on + strand, within Dshi_0339 at 328.186 kb on - strand, within Dshi_0339 at 328.187 kb on - strand, within Dshi_0339 at 328.247 kb on + strand at 328.303 kb on - strand, within Dshi_0339 at 328.389 kb on - strand, within Dshi_0339 at 328.481 kb on + strand at 328.489 kb on - strand at 328.636 kb on - strand at 329.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 327,759 + Dshi_0339 0.11 -3.0 327,786 + Dshi_0339 0.14 +0.1 327,786 + Dshi_0339 0.14 +1.3 327,794 - Dshi_0339 0.15 -2.7 327,794 - Dshi_0339 0.15 -0.7 327,834 - Dshi_0339 0.20 -2.0 327,920 + Dshi_0339 0.30 -0.6 328,186 - Dshi_0339 0.60 +1.4 328,187 - Dshi_0339 0.60 -1.0 328,247 + -2.4 328,303 - Dshi_0339 0.73 -3.0 328,389 - Dshi_0339 0.83 -4.5 328,481 + +1.4 328,489 - -0.8 328,636 - +0.4 329,530 - -0.9
Or see this region's nucleotide sequence