Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0269 and Dshi_0270 are separated by 150 nucleotides Dshi_0270 and Dshi_0271 are separated by 281 nucleotides Dshi_0271 and Dshi_0272 are separated by 12 nucleotides
Dshi_0269: Dshi_0269 - DNA-directed RNA polymerase, beta' subunit (RefSeq), at 269,021 to 273,268
_0269
Dshi_0270: Dshi_0270 - protein of unknown function DUF6 transmembrane (RefSeq), at 273,419 to 274,324
_0270
Dshi_0271: Dshi_0271 - ribosomal protein S12 (RefSeq), at 274,606 to 274,977
_0271
Dshi_0272: Dshi_0272 - ribosomal protein S7 (RefSeq), at 274,990 to 275,460
_0272
Position (kb) 273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 273.138 kb on + strand at 273.372 kb on - strand at 273.622 kb on - strand, within Dshi_0270 at 273.674 kb on + strand, within Dshi_0270 at 273.771 kb on - strand, within Dshi_0270 at 273.787 kb on + strand, within Dshi_0270 at 273.800 kb on + strand, within Dshi_0270 at 273.873 kb on + strand, within Dshi_0270 at 273.988 kb on + strand, within Dshi_0270 at 274.022 kb on - strand, within Dshi_0270 at 274.022 kb on - strand, within Dshi_0270 at 274.180 kb on + strand, within Dshi_0270 at 274.180 kb on + strand, within Dshi_0270 at 274.237 kb on - strand at 274.278 kb on - strand at 274.362 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 273,138 + +1.5 273,372 - -3.4 273,622 - Dshi_0270 0.22 -0.2 273,674 + Dshi_0270 0.28 -0.4 273,771 - Dshi_0270 0.39 +1.5 273,787 + Dshi_0270 0.41 +2.5 273,800 + Dshi_0270 0.42 -0.3 273,873 + Dshi_0270 0.50 +0.9 273,988 + Dshi_0270 0.63 -1.1 274,022 - Dshi_0270 0.67 -0.1 274,022 - Dshi_0270 0.67 +0.9 274,180 + Dshi_0270 0.84 +1.6 274,180 + Dshi_0270 0.84 -0.4 274,237 - +0.0 274,278 - +1.1 274,362 + +0.7
Or see this region's nucleotide sequence