Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0228 and Dshi_0229 are separated by 4 nucleotides Dshi_0229 and Dshi_0230 are separated by 346 nucleotides Dshi_0230 and Dshi_0231 are separated by 12 nucleotides
Dshi_0228: Dshi_0228 - pyruvate kinase (RefSeq), at 225,543 to 226,991
_0228
Dshi_0229: Dshi_0229 - hypothetical protein (RefSeq), at 226,996 to 227,220
_0229
Dshi_0230: Dshi_0230 - ribosomal protein L35 (RefSeq), at 227,567 to 227,767
_0230
Dshi_0231: Dshi_0231 - ribosomal protein L20 (RefSeq), at 227,780 to 228,145
_0231
Position (kb) 226
227
228 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 226.496 kb on + strand, within Dshi_0228 at 226.889 kb on - strand at 227.010 kb on + strand at 227.016 kb on + strand at 227.209 kb on + strand at 227.217 kb on - strand at 227.242 kb on + strand at 227.274 kb on + strand at 227.326 kb on - strand at 227.467 kb on - strand at 228.121 kb on + strand at 228.124 kb on - strand at 228.129 kb on - strand at 228.129 kb on - strand at 228.177 kb on + strand at 228.177 kb on + strand at 228.185 kb on - strand at 228.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 226,496 + Dshi_0228 0.66 -3.3 226,889 - -3.6 227,010 + -2.2 227,016 + +0.1 227,209 + +0.5 227,217 - +0.6 227,242 + -3.4 227,274 + +1.8 227,326 - -0.5 227,467 - +0.0 228,121 + +1.1 228,124 - +0.8 228,129 - +2.5 228,129 - +0.5 228,177 + -1.7 228,177 + +2.5 228,185 - -0.6 228,209 - +1.1
Or see this region's nucleotide sequence