Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0069 and Dshi_0070 are separated by 86 nucleotides Dshi_0070 and Dshi_0071 are separated by 47 nucleotides Dshi_0071 and Dshi_0072 are separated by 61 nucleotides Dshi_0072 and Dshi_0073 are separated by 14 nucleotides
Dshi_0069: Dshi_0069 - Auxin Efflux Carrier (RefSeq), at 69,751 to 70,632
_0069
Dshi_0070: Dshi_0070 - hemimethylated DNA binding protein (RefSeq), at 70,719 to 71,045
_0070
Dshi_0071: Dshi_0071 - Gamma-glutamyltransferase (RefSeq), at 71,093 to 72,676
_0071
Dshi_0072: Dshi_0072 - putative anti-sigma regulatory factor, serine/threonine protein kinase (RefSeq), at 72,738 to 73,247
_0072
Dshi_0073: Dshi_0073 - anti-sigma-factor antagonist (RefSeq), at 73,262 to 73,594
_0073
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 70.157 kb on - strand, within Dshi_0069 at 70.159 kb on + strand, within Dshi_0069 at 70.262 kb on + strand, within Dshi_0069 at 70.270 kb on - strand, within Dshi_0069 at 70.414 kb on + strand, within Dshi_0069 at 70.439 kb on - strand, within Dshi_0069 at 70.441 kb on + strand, within Dshi_0069 at 70.449 kb on - strand, within Dshi_0069 at 70.478 kb on - strand, within Dshi_0069 at 70.489 kb on - strand, within Dshi_0069 at 70.507 kb on + strand, within Dshi_0069 at 70.507 kb on + strand, within Dshi_0069 at 70.539 kb on + strand, within Dshi_0069 at 70.562 kb on - strand at 70.606 kb on + strand at 70.606 kb on + strand at 70.606 kb on + strand at 70.624 kb on - strand at 70.668 kb on + strand at 70.671 kb on - strand at 70.729 kb on - strand at 70.879 kb on + strand, within Dshi_0070 at 71.067 kb on - strand at 71.125 kb on + strand at 71.287 kb on + strand, within Dshi_0071 at 71.411 kb on - strand, within Dshi_0071 at 71.501 kb on + strand, within Dshi_0071 at 71.753 kb on - strand, within Dshi_0071 at 71.763 kb on - strand, within Dshi_0071 at 71.764 kb on - strand, within Dshi_0071 at 71.764 kb on - strand, within Dshi_0071 at 71.847 kb on + strand, within Dshi_0071 at 72.239 kb on + strand, within Dshi_0071 at 72.291 kb on - strand, within Dshi_0071 at 72.382 kb on - strand, within Dshi_0071 at 72.483 kb on - strand, within Dshi_0071 at 72.505 kb on + strand, within Dshi_0071 at 72.592 kb on + strand at 72.682 kb on + strand at 72.756 kb on + strand at 72.759 kb on - strand at 72.855 kb on - strand, within Dshi_0072 at 72.861 kb on - strand, within Dshi_0072 at 72.902 kb on + strand, within Dshi_0072 at 72.903 kb on + strand, within Dshi_0072 at 72.908 kb on + strand, within Dshi_0072 at 72.928 kb on + strand, within Dshi_0072 at 73.005 kb on - strand, within Dshi_0072 at 73.041 kb on - strand, within Dshi_0072 at 73.041 kb on - strand, within Dshi_0072 at 73.046 kb on - strand, within Dshi_0072 at 73.056 kb on - strand, within Dshi_0072 at 73.081 kb on - strand, within Dshi_0072 at 73.099 kb on + strand, within Dshi_0072 at 73.099 kb on + strand, within Dshi_0072 at 73.117 kb on - strand, within Dshi_0072 at 73.120 kb on + strand, within Dshi_0072 at 73.157 kb on - strand, within Dshi_0072 at 73.177 kb on + strand, within Dshi_0072 at 73.210 kb on + strand at 73.210 kb on + strand at 73.210 kb on - strand at 73.212 kb on + strand at 73.218 kb on - strand at 73.392 kb on - strand, within Dshi_0073 at 73.428 kb on - strand, within Dshi_0073 at 73.454 kb on + strand, within Dshi_0073 at 73.455 kb on - strand, within Dshi_0073 at 73.469 kb on + strand, within Dshi_0073 at 73.496 kb on + strand, within Dshi_0073 at 73.538 kb on + strand, within Dshi_0073 at 73.605 kb on + strand at 73.627 kb on + strand at 73.627 kb on + strand at 73.629 kb on - strand at 73.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 70,157 - Dshi_0069 0.46 +1.6 70,159 + Dshi_0069 0.46 -0.2 70,262 + Dshi_0069 0.58 -0.8 70,270 - Dshi_0069 0.59 +2.6 70,414 + Dshi_0069 0.75 +0.9 70,439 - Dshi_0069 0.78 +1.0 70,441 + Dshi_0069 0.78 -2.3 70,449 - Dshi_0069 0.79 -1.4 70,478 - Dshi_0069 0.82 -0.8 70,489 - Dshi_0069 0.84 -1.0 70,507 + Dshi_0069 0.86 +0.8 70,507 + Dshi_0069 0.86 -0.8 70,539 + Dshi_0069 0.89 +0.6 70,562 - +0.8 70,606 + +2.2 70,606 + +2.6 70,606 + -0.0 70,624 - +1.6 70,668 + +2.2 70,671 - -0.1 70,729 - -0.3 70,879 + Dshi_0070 0.49 +2.2 71,067 - +0.1 71,125 + +1.2 71,287 + Dshi_0071 0.12 -0.4 71,411 - Dshi_0071 0.20 -1.0 71,501 + Dshi_0071 0.26 -3.8 71,753 - Dshi_0071 0.42 -0.1 71,763 - Dshi_0071 0.42 -2.0 71,764 - Dshi_0071 0.42 -0.4 71,764 - Dshi_0071 0.42 +0.1 71,847 + Dshi_0071 0.48 +1.0 72,239 + Dshi_0071 0.72 +2.0 72,291 - Dshi_0071 0.76 -2.8 72,382 - Dshi_0071 0.81 -1.1 72,483 - Dshi_0071 0.88 +0.6 72,505 + Dshi_0071 0.89 +0.6 72,592 + +0.6 72,682 + -0.6 72,756 + +2.6 72,759 - +0.2 72,855 - Dshi_0072 0.23 -1.8 72,861 - Dshi_0072 0.24 -2.0 72,902 + Dshi_0072 0.32 -1.8 72,903 + Dshi_0072 0.32 +0.6 72,908 + Dshi_0072 0.33 +0.6 72,928 + Dshi_0072 0.37 +0.1 73,005 - Dshi_0072 0.52 -1.5 73,041 - Dshi_0072 0.59 +0.2 73,041 - Dshi_0072 0.59 -1.2 73,046 - Dshi_0072 0.60 -1.1 73,056 - Dshi_0072 0.62 +0.6 73,081 - Dshi_0072 0.67 +0.9 73,099 + Dshi_0072 0.71 -0.4 73,099 + Dshi_0072 0.71 -0.4 73,117 - Dshi_0072 0.74 -1.8 73,120 + Dshi_0072 0.75 -0.9 73,157 - Dshi_0072 0.82 -1.4 73,177 + Dshi_0072 0.86 -1.4 73,210 + -0.5 73,210 + -1.4 73,210 - -2.0 73,212 + -0.0 73,218 - -0.4 73,392 - Dshi_0073 0.39 +1.6 73,428 - Dshi_0073 0.50 +3.6 73,454 + Dshi_0073 0.58 +0.6 73,455 - Dshi_0073 0.58 +0.6 73,469 + Dshi_0073 0.62 +0.2 73,496 + Dshi_0073 0.70 -2.6 73,538 + Dshi_0073 0.83 +0.2 73,605 + +1.6 73,627 + -1.0 73,627 + +3.4 73,629 - +2.5 73,635 - -2.6
Or see this region's nucleotide sequence