Experiment: MoYLS4 with G418 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0831 and DvMF_0832 are separated by 151 nucleotides DvMF_0832 and DvMF_0833 are separated by 278 nucleotides DvMF_0833 and DvMF_0834 overlap by 1 nucleotides DvMF_0834 and DvMF_0835 are separated by 133 nucleotides
DvMF_0831: DvMF_0831 - protein of unknown function DUF21 (RefSeq), at 1,003,800 to 1,005,158
_0831
DvMF_0832: DvMF_0832 - hypothetical protein (RefSeq), at 1,005,310 to 1,005,576
_0832
DvMF_0833: DvMF_0833 - cobalamin biosynthesis protein CbiB (RefSeq), at 1,005,855 to 1,006,979
_0833
DvMF_0834: DvMF_0834 - tRNA/rRNA methyltransferase (SpoU) (RefSeq), at 1,006,979 to 1,007,437
_0834
DvMF_0835: DvMF_0835 - Beta-N-acetylhexosaminidase (RefSeq), at 1,007,571 to 1,009,331
_0835
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1005.042 kb on - strand at 1005.238 kb on + strand at 1005.246 kb on - strand at 1005.436 kb on + strand, within DvMF_0832 at 1005.705 kb on + strand at 1005.723 kb on - strand at 1005.795 kb on + strand at 1005.838 kb on - strand at 1006.609 kb on + strand, within DvMF_0833 at 1006.635 kb on + strand, within DvMF_0833 at 1007.047 kb on + strand, within DvMF_0834 at 1007.047 kb on + strand, within DvMF_0834 at 1007.047 kb on + strand, within DvMF_0834 at 1007.055 kb on - strand, within DvMF_0834 at 1007.055 kb on - strand, within DvMF_0834 at 1007.055 kb on - strand, within DvMF_0834 at 1007.055 kb on - strand, within DvMF_0834 at 1007.055 kb on - strand, within DvMF_0834 at 1007.118 kb on - strand, within DvMF_0834 at 1007.951 kb on - strand, within DvMF_0835
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with G418 0.125 mg/ml remove 1,005,042 - -0.4 1,005,238 + -0.5 1,005,246 - -0.5 1,005,436 + DvMF_0832 0.47 -0.6 1,005,705 + -0.6 1,005,723 - -0.2 1,005,795 + -0.1 1,005,838 - -0.3 1,006,609 + DvMF_0833 0.67 -2.1 1,006,635 + DvMF_0833 0.69 -4.4 1,007,047 + DvMF_0834 0.15 -2.6 1,007,047 + DvMF_0834 0.15 -3.3 1,007,047 + DvMF_0834 0.15 -2.4 1,007,055 - DvMF_0834 0.17 -3.1 1,007,055 - DvMF_0834 0.17 -2.4 1,007,055 - DvMF_0834 0.17 -2.1 1,007,055 - DvMF_0834 0.17 -1.3 1,007,055 - DvMF_0834 0.17 -4.3 1,007,118 - DvMF_0834 0.30 -0.7 1,007,951 - DvMF_0835 0.22 -0.7
Or see this region's nucleotide sequence