Experiment: MoYLS4 with G418 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0202 and DvMF_0203 are separated by 237 nucleotides DvMF_0203 and DvMF_0204 are separated by 156 nucleotides
DvMF_0202: DvMF_0202 - phosphoribosylaminoimidazole synthetase (RefSeq), at 219,857 to 220,909
_0202
DvMF_0203: DvMF_0203 - Radical SAM domain protein (RefSeq), at 221,147 to 222,682
_0203
DvMF_0204: DvMF_0204 - hypothetical protein (RefSeq), at 222,839 to 223,672
_0204
Position (kb) 221
222
223 Strain fitness (log2 ratio) -2
-1
0
1 at 220.270 kb on + strand, within DvMF_0202 at 220.816 kb on - strand at 220.979 kb on + strand at 220.992 kb on - strand at 220.992 kb on - strand at 220.992 kb on - strand at 221.002 kb on - strand at 221.110 kb on + strand at 221.118 kb on - strand at 221.230 kb on - strand at 221.322 kb on - strand, within DvMF_0203 at 221.796 kb on - strand, within DvMF_0203 at 222.029 kb on + strand, within DvMF_0203 at 222.148 kb on + strand, within DvMF_0203 at 222.264 kb on - strand, within DvMF_0203 at 222.279 kb on + strand, within DvMF_0203 at 222.287 kb on - strand, within DvMF_0203 at 222.332 kb on - strand, within DvMF_0203 at 222.384 kb on - strand, within DvMF_0203 at 222.504 kb on - strand, within DvMF_0203 at 222.860 kb on + strand at 222.877 kb on + strand at 222.932 kb on - strand, within DvMF_0204 at 222.970 kb on + strand, within DvMF_0204 at 222.970 kb on + strand, within DvMF_0204 at 222.995 kb on - strand, within DvMF_0204 at 223.298 kb on - strand, within DvMF_0204 at 223.346 kb on + strand, within DvMF_0204 at 223.371 kb on + strand, within DvMF_0204 at 223.448 kb on + strand, within DvMF_0204 at 223.556 kb on + strand, within DvMF_0204
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with G418 0.125 mg/ml remove 220,270 + DvMF_0202 0.39 -2.4 220,816 - -1.2 220,979 + -0.2 220,992 - -0.1 220,992 - -0.9 220,992 - -0.5 221,002 - -0.3 221,110 + -0.6 221,118 - +0.3 221,230 - +0.2 221,322 - DvMF_0203 0.11 -0.2 221,796 - DvMF_0203 0.42 +0.1 222,029 + DvMF_0203 0.57 -0.6 222,148 + DvMF_0203 0.65 -0.6 222,264 - DvMF_0203 0.73 -0.1 222,279 + DvMF_0203 0.74 +0.8 222,287 - DvMF_0203 0.74 -0.3 222,332 - DvMF_0203 0.77 -0.6 222,384 - DvMF_0203 0.81 -1.4 222,504 - DvMF_0203 0.88 -0.5 222,860 + -0.2 222,877 + -0.2 222,932 - DvMF_0204 0.11 -0.0 222,970 + DvMF_0204 0.16 -0.5 222,970 + DvMF_0204 0.16 -0.3 222,995 - DvMF_0204 0.19 -0.2 223,298 - DvMF_0204 0.55 +0.4 223,346 + DvMF_0204 0.61 -0.2 223,371 + DvMF_0204 0.64 -0.2 223,448 + DvMF_0204 0.73 -0.3 223,556 + DvMF_0204 0.86 -0.2
Or see this region's nucleotide sequence