Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3499 and Shew_3500 are separated by 34 nucleotides Shew_3500 and Shew_3501 are separated by 68 nucleotides Shew_3501 and Shew_3502 are separated by 341 nucleotides
Shew_3499: Shew_3499 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,184,648 to 4,186,753
_3499
Shew_3500: Shew_3500 - putative endoribonuclease L-PSP (RefSeq), at 4,186,788 to 4,187,171
_3500
Shew_3501: Shew_3501 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 4,187,240 to 4,187,944
_3501
Shew_3502: Shew_3502 - AMP-dependent synthetase and ligase (RefSeq), at 4,188,286 to 4,189,941
_3502
Position (kb)
4187
4188 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4186.480 kb on + strand, within Shew_3499 at 4186.560 kb on - strand at 4187.011 kb on - strand, within Shew_3500 at 4187.095 kb on - strand, within Shew_3500 at 4187.449 kb on + strand, within Shew_3501 at 4187.771 kb on - strand, within Shew_3501 at 4187.803 kb on + strand, within Shew_3501 at 4187.803 kb on + strand, within Shew_3501 at 4187.811 kb on - strand, within Shew_3501 at 4187.813 kb on + strand, within Shew_3501 at 4187.818 kb on + strand, within Shew_3501 at 4188.273 kb on - strand at 4188.297 kb on + strand at 4188.414 kb on - strand at 4188.452 kb on + strand, within Shew_3502 at 4188.460 kb on - strand, within Shew_3502 at 4188.507 kb on - strand, within Shew_3502 at 4188.535 kb on + strand, within Shew_3502 at 4188.552 kb on + strand, within Shew_3502 at 4188.774 kb on - strand, within Shew_3502 at 4188.783 kb on + strand, within Shew_3502 at 4188.822 kb on + strand, within Shew_3502 at 4188.843 kb on - strand, within Shew_3502 at 4188.843 kb on - strand, within Shew_3502 at 4188.885 kb on + strand, within Shew_3502
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 4,186,480 + Shew_3499 0.87 +0.7 4,186,560 - +0.7 4,187,011 - Shew_3500 0.58 +0.6 4,187,095 - Shew_3500 0.80 +0.5 4,187,449 + Shew_3501 0.30 +0.6 4,187,771 - Shew_3501 0.75 -0.1 4,187,803 + Shew_3501 0.80 -0.3 4,187,803 + Shew_3501 0.80 -0.1 4,187,811 - Shew_3501 0.81 -0.0 4,187,813 + Shew_3501 0.81 +0.4 4,187,818 + Shew_3501 0.82 +0.0 4,188,273 - +0.6 4,188,297 + +1.6 4,188,414 - +0.1 4,188,452 + Shew_3502 0.10 +0.1 4,188,460 - Shew_3502 0.11 -0.4 4,188,507 - Shew_3502 0.13 -0.3 4,188,535 + Shew_3502 0.15 -0.2 4,188,552 + Shew_3502 0.16 +2.9 4,188,774 - Shew_3502 0.29 -1.0 4,188,783 + Shew_3502 0.30 -0.0 4,188,822 + Shew_3502 0.32 +0.5 4,188,843 - Shew_3502 0.34 +0.1 4,188,843 - Shew_3502 0.34 -0.4 4,188,885 + Shew_3502 0.36 -1.5
Or see this region's nucleotide sequence