Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2535 and Shew_2536 are separated by 40 nucleotides Shew_2536 and Shew_2537 are separated by 307 nucleotides Shew_2537 and Shew_2538 are separated by 216 nucleotides Shew_2538 and Shew_2539 overlap by 4 nucleotides
Shew_2535: Shew_2535 - pyridoxal-dependent decarboxylase (RefSeq), at 2,992,865 to 2,994,505
_2535
Shew_2536: Shew_2536 - putative thiol-disulphide oxidoreductase DCC (RefSeq), at 2,994,546 to 2,995,016
_2536
Shew_2537: Shew_2537 - AraC family transcriptional regulator (RefSeq), at 2,995,324 to 2,996,262
_2537
Shew_2538: Shew_2538 - AzlC family protein (RefSeq), at 2,996,479 to 2,997,216
_2538
Shew_2539: Shew_2539 - branched-chain amino acid transport (RefSeq), at 2,997,213 to 2,997,518
_2539
Position (kb)
2995
2996
2997 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2994.378 kb on + strand at 2994.655 kb on + strand, within Shew_2536 at 2994.737 kb on + strand, within Shew_2536 at 2994.958 kb on + strand, within Shew_2536 at 2995.031 kb on + strand at 2995.127 kb on - strand at 2995.157 kb on + strand at 2995.435 kb on - strand, within Shew_2537 at 2995.519 kb on - strand, within Shew_2537 at 2995.668 kb on - strand, within Shew_2537 at 2996.405 kb on - strand at 2996.748 kb on + strand, within Shew_2538 at 2996.804 kb on - strand, within Shew_2538 at 2996.960 kb on + strand, within Shew_2538 at 2997.043 kb on - strand, within Shew_2538 at 2997.086 kb on + strand, within Shew_2538 at 2997.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 2,994,378 + +3.0 2,994,655 + Shew_2536 0.23 -0.0 2,994,737 + Shew_2536 0.41 +1.2 2,994,958 + Shew_2536 0.87 +1.6 2,995,031 + +0.1 2,995,127 - -2.6 2,995,157 + +0.3 2,995,435 - Shew_2537 0.12 -0.9 2,995,519 - Shew_2537 0.21 -0.6 2,995,668 - Shew_2537 0.37 -0.2 2,996,405 - -0.8 2,996,748 + Shew_2538 0.36 -0.4 2,996,804 - Shew_2538 0.44 +0.1 2,996,960 + Shew_2538 0.65 +0.2 2,997,043 - Shew_2538 0.76 +0.5 2,997,086 + Shew_2538 0.82 -1.3 2,997,148 + +0.5
Or see this region's nucleotide sequence