Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4199 and Dshi_4200 are separated by 145 nucleotides Dshi_4200 and Dshi_4201 overlap by 4 nucleotides Dshi_4201 and Dshi_4202 overlap by 4 nucleotides
Dshi_4199: Dshi_4199 - transcriptional regulator, TetR family (RefSeq), at 45,953 to 46,597
_4199
Dshi_4200: Dshi_4200 - Carbon-monoxide dehydrogenase (acceptor) (RefSeq), at 46,743 to 47,576
_4200
Dshi_4201: Dshi_4201 - (2Fe-2S)-binding domain protein (RefSeq), at 47,573 to 48,073
_4201
Dshi_4202: Dshi_4202 - Carbon-monoxide dehydrogenase (acceptor) (RefSeq), at 48,070 to 50,451
_4202
Position (kb) 47
48
49 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 46.771 kb on + strand at 46.982 kb on + strand, within Dshi_4200 at 47.297 kb on + strand, within Dshi_4200 at 47.316 kb on - strand, within Dshi_4200 at 47.331 kb on + strand, within Dshi_4200 at 47.589 kb on - strand at 47.630 kb on + strand, within Dshi_4201 at 47.638 kb on + strand, within Dshi_4201 at 47.638 kb on - strand, within Dshi_4201 at 47.651 kb on + strand at 47.690 kb on - strand, within Dshi_4201 at 47.816 kb on + strand, within Dshi_4201 at 48.159 kb on - strand at 48.231 kb on - strand at 48.328 kb on + strand, within Dshi_4202 at 48.348 kb on - strand, within Dshi_4202
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 46,771 + +2.8 46,982 + Dshi_4200 0.29 +1.8 47,297 + Dshi_4200 0.66 -0.8 47,316 - Dshi_4200 0.69 -0.2 47,331 + Dshi_4200 0.71 -2.2 47,589 - -1.1 47,630 + Dshi_4201 0.11 -0.4 47,638 + Dshi_4201 0.13 -0.8 47,638 - Dshi_4201 0.13 +0.2 47,651 + +1.8 47,690 - Dshi_4201 0.23 +2.4 47,816 + Dshi_4201 0.49 -3.1 48,159 - +1.1 48,231 - -2.8 48,328 + Dshi_4202 0.11 -1.2 48,348 - Dshi_4202 0.12 +0.8
Or see this region's nucleotide sequence