Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4119 and Dshi_4120 overlap by 4 nucleotides Dshi_4120 and Dshi_4121 are separated by 147 nucleotides Dshi_4121 and Dshi_4122 overlap by 4 nucleotides
Dshi_4119: Dshi_4119 - NAD-dependent epimerase/dehydratase (RefSeq), at 50,748 to 51,629
_4119
Dshi_4120: Dshi_4120 - sugar transferase (RefSeq), at 51,626 to 52,234
_4120
Dshi_4121: Dshi_4121 - glycosyl transferase family 2 (RefSeq), at 52,382 to 54,934
_4121
Dshi_4122: Dshi_4122 - hypothetical protein (RefSeq), at 54,931 to 55,707
_4122
Position (kb) 52
53
54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 52.118 kb on + strand, within Dshi_4120 at 52.146 kb on + strand, within Dshi_4120 at 52.273 kb on + strand at 52.306 kb on - strand at 52.390 kb on + strand at 52.489 kb on - strand at 52.631 kb on + strand at 52.822 kb on - strand, within Dshi_4121 at 52.832 kb on + strand, within Dshi_4121 at 52.857 kb on - strand, within Dshi_4121 at 52.862 kb on - strand, within Dshi_4121 at 53.008 kb on - strand, within Dshi_4121 at 53.014 kb on - strand, within Dshi_4121 at 53.048 kb on + strand, within Dshi_4121 at 53.056 kb on - strand, within Dshi_4121 at 53.056 kb on - strand, within Dshi_4121 at 53.056 kb on - strand, within Dshi_4121 at 53.062 kb on + strand, within Dshi_4121 at 53.623 kb on + strand, within Dshi_4121 at 53.722 kb on - strand, within Dshi_4121 at 53.769 kb on + strand, within Dshi_4121 at 54.077 kb on - strand, within Dshi_4121 at 54.346 kb on - strand, within Dshi_4121 at 54.360 kb on + strand, within Dshi_4121 at 54.387 kb on - strand, within Dshi_4121 at 54.581 kb on - strand, within Dshi_4121 at 54.625 kb on + strand, within Dshi_4121 at 54.637 kb on + strand, within Dshi_4121 at 54.693 kb on + strand at 54.738 kb on + strand at 54.777 kb on - strand at 54.972 kb on + strand at 55.039 kb on + strand, within Dshi_4122 at 55.196 kb on + strand, within Dshi_4122 at 55.264 kb on - strand, within Dshi_4122 at 55.328 kb on + strand, within Dshi_4122 at 55.337 kb on + strand, within Dshi_4122 at 55.487 kb on + strand, within Dshi_4122 at 55.559 kb on + strand, within Dshi_4122 at 55.560 kb on + strand, within Dshi_4122 at 55.687 kb on + strand at 55.736 kb on - strand at 55.747 kb on - strand at 55.760 kb on - strand at 55.827 kb on - strand at 55.837 kb on + strand at 55.839 kb on + strand at 55.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 52,118 + Dshi_4120 0.81 -1.1 52,146 + Dshi_4120 0.85 -0.7 52,273 + -1.6 52,306 - -1.7 52,390 + -1.3 52,489 - -1.9 52,631 + -1.8 52,822 - Dshi_4121 0.17 -2.1 52,832 + Dshi_4121 0.18 +0.3 52,857 - Dshi_4121 0.19 -0.5 52,862 - Dshi_4121 0.19 -3.7 53,008 - Dshi_4121 0.25 -2.1 53,014 - Dshi_4121 0.25 +2.3 53,048 + Dshi_4121 0.26 -1.6 53,056 - Dshi_4121 0.26 +0.3 53,056 - Dshi_4121 0.26 -1.9 53,056 - Dshi_4121 0.26 -0.4 53,062 + Dshi_4121 0.27 -1.5 53,623 + Dshi_4121 0.49 +0.3 53,722 - Dshi_4121 0.52 +0.8 53,769 + Dshi_4121 0.54 -0.7 54,077 - Dshi_4121 0.66 -2.1 54,346 - Dshi_4121 0.77 -2.3 54,360 + Dshi_4121 0.77 -1.0 54,387 - Dshi_4121 0.79 -2.2 54,581 - Dshi_4121 0.86 -1.6 54,625 + Dshi_4121 0.88 -1.7 54,637 + Dshi_4121 0.88 -2.7 54,693 + -0.7 54,738 + -1.2 54,777 - -3.3 54,972 + +0.4 55,039 + Dshi_4122 0.14 +0.5 55,196 + Dshi_4122 0.34 +1.3 55,264 - Dshi_4122 0.43 +0.8 55,328 + Dshi_4122 0.51 -2.5 55,337 + Dshi_4122 0.52 -0.4 55,487 + Dshi_4122 0.72 +1.3 55,559 + Dshi_4122 0.81 -3.4 55,560 + Dshi_4122 0.81 +0.5 55,687 + -0.7 55,736 - +1.0 55,747 - +2.6 55,760 - -0.3 55,827 - -1.5 55,837 + +1.6 55,839 + -2.9 55,847 - -1.1
Or see this region's nucleotide sequence