Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_R0050 and Dshi_3228 are separated by 96 nucleotides Dshi_3228 and Dshi_3229 overlap by 4 nucleotides Dshi_3229 and Dshi_3230 are separated by 23 nucleotides Dshi_3230 and Dshi_3231 are separated by 211 nucleotides
Dshi_R0050: Dshi_R0050 - tRNA-Lys (RefSeq), at 3,396,915 to 3,396,990
_R0050
Dshi_3228: Dshi_3228 - Diaminopimelate epimerase (RefSeq), at 3,397,087 to 3,397,935
_3228
Dshi_3229: Dshi_3229 - MiaB-like tRNA modifying enzyme (RefSeq), at 3,397,932 to 3,399,182
_3229
Dshi_3230: Dshi_3230 - hypothetical protein (RefSeq), at 3,399,206 to 3,399,451
_3230
Dshi_3231: Dshi_3231 - hypothetical protein (RefSeq), at 3,399,663 to 3,401,321
_3231
Position (kb)
3397
3398
3399
3400 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3397.075 kb on + strand at 3397.982 kb on + strand at 3397.982 kb on - strand at 3397.990 kb on - strand at 3398.150 kb on + strand, within Dshi_3229 at 3398.383 kb on - strand, within Dshi_3229 at 3398.487 kb on + strand, within Dshi_3229 at 3398.523 kb on + strand, within Dshi_3229 at 3398.577 kb on + strand, within Dshi_3229 at 3398.625 kb on - strand, within Dshi_3229 at 3398.709 kb on - strand, within Dshi_3229 at 3398.923 kb on - strand, within Dshi_3229 at 3399.033 kb on - strand, within Dshi_3229 at 3399.079 kb on + strand at 3399.084 kb on + strand at 3399.092 kb on - strand at 3399.334 kb on - strand, within Dshi_3230 at 3399.385 kb on - strand, within Dshi_3230 at 3399.385 kb on - strand, within Dshi_3230 at 3399.572 kb on + strand at 3399.579 kb on - strand at 3399.603 kb on - strand at 3399.690 kb on - strand at 3399.715 kb on + strand at 3399.791 kb on - strand at 3399.851 kb on - strand, within Dshi_3231 at 3399.876 kb on + strand, within Dshi_3231 at 3399.879 kb on - strand, within Dshi_3231 at 3400.066 kb on + strand, within Dshi_3231
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 3,397,075 + -2.1 3,397,982 + -1.6 3,397,982 - +0.4 3,397,990 - -1.2 3,398,150 + Dshi_3229 0.17 -0.2 3,398,383 - Dshi_3229 0.36 -2.2 3,398,487 + Dshi_3229 0.44 -0.2 3,398,523 + Dshi_3229 0.47 +0.4 3,398,577 + Dshi_3229 0.52 -1.3 3,398,625 - Dshi_3229 0.55 -0.6 3,398,709 - Dshi_3229 0.62 -2.6 3,398,923 - Dshi_3229 0.79 -1.6 3,399,033 - Dshi_3229 0.88 +0.8 3,399,079 + +0.4 3,399,084 + +2.4 3,399,092 - -2.6 3,399,334 - Dshi_3230 0.52 -0.9 3,399,385 - Dshi_3230 0.73 -2.3 3,399,385 - Dshi_3230 0.73 -0.7 3,399,572 + -0.6 3,399,579 - -2.4 3,399,603 - +0.4 3,399,690 - -1.8 3,399,715 + -1.2 3,399,791 - -0.1 3,399,851 - Dshi_3231 0.11 -0.3 3,399,876 + Dshi_3231 0.13 -2.2 3,399,879 - Dshi_3231 0.13 -0.3 3,400,066 + Dshi_3231 0.24 -2.2
Or see this region's nucleotide sequence