Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3100 and Dshi_3101 are separated by 60 nucleotides Dshi_3101 and Dshi_3102 overlap by 4 nucleotides Dshi_3102 and Dshi_3103 are separated by 7 nucleotides Dshi_3103 and Dshi_3104 are separated by 1 nucleotides
Dshi_3100: Dshi_3100 - 4-hydroxybenzoate polyprenyl transferase (RefSeq), at 3,267,233 to 3,268,201
_3100
Dshi_3101: Dshi_3101 - OmpA/MotB domain protein (RefSeq), at 3,268,262 to 3,270,115
_3101
Dshi_3102: Dshi_3102 - hypothetical protein (RefSeq), at 3,270,112 to 3,270,444
_3102
Dshi_3103: Dshi_3103 - molybdopterin biosynthesis MoaE protein (RefSeq), at 3,270,452 to 3,270,895
_3103
Dshi_3104: Dshi_3104 - molybdopterin converting factor, subunit 1 (RefSeq), at 3,270,897 to 3,271,148
_3104
Position (kb) 3268
3269
3270
3271 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3268.334 kb on - strand at 3268.753 kb on - strand, within Dshi_3101 at 3269.335 kb on + strand, within Dshi_3101 at 3270.176 kb on + strand, within Dshi_3102 at 3270.394 kb on + strand, within Dshi_3102 at 3270.395 kb on + strand, within Dshi_3102 at 3270.823 kb on - strand, within Dshi_3103 at 3271.101 kb on + strand, within Dshi_3104
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 3,268,334 - -1.8 3,268,753 - Dshi_3101 0.26 +1.5 3,269,335 + Dshi_3101 0.58 +0.9 3,270,176 + Dshi_3102 0.19 -1.1 3,270,394 + Dshi_3102 0.85 -2.1 3,270,395 + Dshi_3102 0.85 +0.5 3,270,823 - Dshi_3103 0.84 -2.8 3,271,101 + Dshi_3104 0.81 +0.3
Or see this region's nucleotide sequence